Protein Expression
NEB’s portfolio of protein expression products includes a wide variety of tools, included our new NEBExpress® portfolio, and is supported by access to scientists with over 40 years of experience in developing and using recombinant protein technologies in E. coli. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical – all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours. Protein Expression at NEB
New England Biolabs is committed to providing advanced tools for protein expression. As a leading supplier for reagents in the biotechnology field, NEB understands the importance of convenient and easy-to-use systems for high level expression and purification of proteins. We invite you to review our growing range of expression systems, including our new line of NEBExpress® products, resulting from our 50+ years of experience in cloning, over-expression and purification. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical – all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours.
Choose Product:
E. coli Protein Expression Strains
Cell-Free Protein Expression
Bacterial E. coli Protein Expression
Protein Expression in Yeast
T7 Expression
Non-T7 Expression
SHuffle Expression
NEBExpress® Cell-free E. coli Protein Synthesis System
PURExpress® In Vitro Protein Synthesis
NEBExpress MBP Fusion and Purification System
IMPACT System
Choose Type:
- Quick Start Protocol for NEBExpress® Cell-free E. coli Protein Synthesis System
- Protein Synthesis Reaction using PURExpress (E6800)
- NEBExpress MBP Fusion and Purification System Quick Start Protocol (NEB #E8201)
- Simplified Expression and Purification Protocol (E6901)
- Protein Expression using the K. lactis Protein Expression Kit - Simultaneous Expression of Multiple Proteins
- Transformation of SHuffle® Competent Cell Strains
- E coli Lemo21 DE3 A T7 RNA Polymerase-based protein overexpression platform for routine and difficult targets
- Co-expression of Multiple Proteins in Kluyveromyces lactis
- Protein Expression with T7 Express Strains
- Use of the PURExpress® in vitro Protein Synthesis Kit, Disulfide Bond Enhancer and SHuffle® Competent E. coli for heterologous in vitro and in vivo cellulase expression.
- Using the PURExpress® In Vitro Protein Synthesis Kit for Heterologous In Vitro Expression and Functional Screening of FMN-dependent Oxidoreductase Variants
- NEBExpress® Cell-free E. coli Protein Synthesis System
- Scaling down to scale up – Miniaturizing cell-free protein synthesis reactions with the Echo 525 Acoustic Liquid Handler
- Automated Cell-free Protein Expression and Purification for High-Throughput Screening using NEBExpress® Cell-free E. coli Protein Synthesis System and NEBExpress Ni-NTA Magnetic Beads
- Cell-free Expression and Rapid Analysis of Single-Chain Antibody Fragments
- From DNA Template to BLI Kinetics in a Day - Using Octet® VHH Biosensors to Directly Analyze VHHs Produced in NEBExpress® and PURExpress® Cell-Free Protein Synthesis Systems
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Avoid Common Obstacles in Protein Expression
Read how to avoid common obstacles in protein expression that prevent interactions with cellular machinery.
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The Future of Cell-Free Protein Synthesis
Cell-free protein synthesis has the potential to become one of the most important high throughput technologies for functional genomics and proteomics.
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Why Choose the K. lactis Protein Expression Kit?
Review the advantages of the K. lactis Protein Expression Kit for rapid, high yield protein expression in yeast.
- Purification Beads, Columns and Resins Brochure
- Competent Cell Brochure
- Protein Expression & Purification Brochure
- Protein Expression and Purification Selection Chart
- IMPACT™ Vectors and Applications
- Competent Cell Product Comparison
- NiCo21(DE3): A BL21(DE3) Derivative Designed for Expression and Purification of His-tagged Recombinant Protein (2015)
- Development of a Rapid Method for Genome Engineering in a Quest Towards Customized Protein Expression Strains (2015)
- Discovering the Maltose Binding Protein epitope tag for Escherichia coli expressed proteins (2018)
- An E.coli Cell Lysate Based System for in vitro Protein Synthesis
- An E.coli lysate-based system for in vitro Protein Synthesis
- Cell-Free Production of Antibody Fragments for High-Throughput Analyses
- Automated Protein Expression and Purification of NEBExpress® Cell-free E. coli Protein Synthesis Reactions using NEBExpress Ni-NTA Magnetic Beads
- Reproducible performance, versatility, and point-of-use optimization of NEBExpress® Cell-free E. coli Protein Synthesis System
Feature Articles
Brochures
Selection Tools
Posters
- Bhat, R. K. and Berger, S. (2007) New and easy strategy for cloning, expression, purification, and characterization of the 5S subunit of transcarboxylase from Propionibacterium f. shermanii Prep Biochem Biotechnol; 37, 13-26. PubMedID: 17134979
Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. All other trademarks are the property of their respective owners. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
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