Description
IMPACT Citations
pTXB1 is an E. coli expression vector in the IMPACT™ Kit (NEB #E6901) (1,2). It is designed for the in-frame insertion of a target gene into the polylinker upstream of the Mxe intein/chitin binding domain (27 kDa) (2,3,4,5). The fusion protein is bound to chitin beads and the thiol-induced cleavage activity of the intein releases the target protein. pTXB vectors are recommended for use in intein-mediated protein ligation and C-terminal labeling (2). This double stranded vector is 6,706 base pairs in length.
DNASU and Addgene are central repositories for plasmid clones and collections that may also be helpful.
- This product is related to the following categories:
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IMPACT System,
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Bacterial E. coli Protein Expression Products,
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DNA Plasmids & Substrates Products,
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Chitin Purification (CBD-tag),
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Protein Purification Products,
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Protein Expression Products,
- This product can be used in the following applications:
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Protein Expression in E. Coli,
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Protein Expression