Product Class: Other

E. coli Ribosome
Gray property icon for heat inactivation no

Product Introduction

The E. coli 70S ribosome consists of the large 50S and the small 30S subunits.  

  • Highly active in the PURExpress® In vitro Protein Synthesis Kit (NEB #E6800)
  • Purified from an E. coli B strain
  • Ideal for ribosome structural and functional studies
  • Target for drug screening
Bioz Badge Exists : True
Catalog # Size Concentration
P0763S 1 mg 13.3 µM

Product Information

Description

The ribosome is the machinery for protein synthesis in a cell. The 70S ribosome consists of a small subunit (30S) and a large subunit (50S). This preparation of ribosome is highly active in the PURExpress Protein Synthesis Kit (NEB #E6800).

Product Source

The ribosome is purified from an E. coli B strain
This product is related to the following categories:
PURExpress® In Vitro Protein Synthesis,
Cell-Free Protein Expression,
Protein Expression,
This product can be used in the following applications:
PURExpress,
Cell-Free Protein Expression,
Protein Expression

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Heat Inactivation

No

Product Notes

  1. Avoid multiple freeze/thaw cycles. If you plan to use the ribosome multiple times, we recommend preparing aliquots after first thaw. Handle the ribosome gently, mix by tapping the tube, and avoid vigorous vortexing or high speed centrifugation. Mg2+ is necessary to keep the ribosome intact. If you are going to dilute the ribosome, be sure to include 10 mM magnesium acetate in the buffer.
  2. In vitro translation
  3. Ribosome structural and functional studies
  4. Target for drug screening
  5. Starting material for isolation of native ribosomal RNAs (5S, 16S and 23S)

FAQs & Troubleshooting

FAQs

  1. What is the storage buffer for the E. coli ribosomes?
  2. Are the ribosomes the same as what is provided in the PURExpress Protein Synthesis kits?
  3. I want to work with the 50S or 30S ribosomal subunits. Are the 50S and 30S ribosomal subunits available dissociated/separately?
  4. How many PURExpress reactions in one pack of E. coli ribosome P0763S?
  5. Where can I find many more detailed FAQs for PURExpress?

Tech Tips

Do not vortex to mix, mix gently
Avoid freeze/thaw cycles

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In