Product Class: Other

K. lactis GG799 Competent Cells

Product Introduction

Chemically competent Kluyveromyces lactis cells suitable for transformation with a linearized pKLAC1-based expression vector. GG799 cells are characterized by very high cell density growth and efficient expression of foreign proteins.

  • Sterile – competent cells are free of bacterial and fungal contamination.
  • Verified Transformation Efficiency - competent cells are tested for transformation efficiency as defined by the number of colony forming units (cfu) produced by transforming 1 μg of plasmid into a given volume of competent cells.
  • Optimal stability and efficiency if stored at -80C for up to 12 months.
  • GG799 cells have no genetic modifications.
  • GG799 is the basic strain provided in the K. lactis Protein Expression Kit (NEB #E1000S)
  • NEB does not add a dry ice surcharge to our competent cell shipments.

 

Bioz Badge Exists : True
Catalog # Size Concentration
C1001S 5 reactions

Product Information

Description

This product is related to the following categories:
Yeast Expression Strains,
Protein Expression in Yeast,
Protein Expression,
This product can be used in the following applications:
Protein Expression in Yeast ,
Protein Expression

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    C1001S -80
    Component Name Component # Stored at (°C) Amount Concentration
    K. lactis GG799 Competent Cells C1001SVIAL -80 5 x 0.2 ml Not Applicable
    NEB® Yeast Transformation Reagent (5 ml) M2570SVIAL 4 1 x 5 ml Not Applicable

Properties & Usage

Shipping Notes

  • Ships on dry ice

Product Notes

  1. Store Competent Cells at -80°C. Once Thawed, Do Not Re-freeze.
  2. Due to the high transformation efficiency of K. lactis GG799 Competent Cells, plating multiple dilutions of the cell mixture is necessary to ensure formation of plates with distinct single colonies. Growth time should not exceed 5 days as small colonies that lack an integrated expression fragment may form.
  3. Plates containing colonies can be stored at 4°C for up to two weeks.

FAQs & Troubleshooting

Tech Tips

Always store immediately at -80 C.  Yeast competent cells will quickly lose competency if handled improperly.  

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Licenses

Notice to Buyer/User: K. lactis Competent Cells are a component of an expression system that was developed from basic research at New England Biolabs and DSM Biologics Company B.V. The buyer and user has a non-exclusive sublicense to use this system or any component thereof, including the K. lactis GG799, YCT284, YCT569, YCT389, YCT390 and YCT598 Competent Cells, for RESEARCH PURPOSES ONLY. A license to use this system for manufacture of clinical grade material or commercial purposes is available from New England Biolabs, Inc., or DSM Biologics Company B.V.

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