Product Class: Other

Goat Anti-Rabbit IgG Magnetic Beads

This product was discontinued on 12/15/2021. Alternate products include Protein A Magnetic Beads (NEB #S1425) and/or Protein G Magnetic Beads (NEB #S1430). Please see selection chart for details.

  • Catalog # S1432 was discontinued on December 15, 2021

Product Introduction

An affinity matrix for the small-scale immunomagnetic separation and purification of rabbit IgGs. Specifically, the beads consist of Goat Anti-Rabbit IgG that is covalently coupled to a 1 μm nonporous superparamagnetic particle.

  • This secondary antibody binds the heavy chain of all rabbit IgG subclasses and is suitable for immunoassays that employ a rabbit IgG primary polyclonal antibody
  • Cell separations and sorting can be accomplished using a rabbit IgG antibody to defined cell surface antigens
  • Binding capacity: 5 μg rabbit lgG/mg Goat Anti-Rabbit IgG magnetic beads
  • Quick, small-scale purification of rabbit IgGs affords higher throughput and efficiency
Bioz Badge Exists : True
Catalog # Size Concentration

Product Information

Description

An affinity matrix for the small-scale immunomagnetic separation and purification of rabbit IgG's. Goat Anti-Rabbit IgG is covalently coupled to a nonporous superparamagnetic particle. This secondary antibody binds the heavy chain of all rabbit IgG subclasses and is suitable for immunoassays that employ a rabbit IgG primary polyclonal antibody. Cell separations and sorting can be accomplished using a rabbit IgG antibody to defined cell surface antigens.

Supplied as a 1 ml suspension in PBS Buffer (pH 7.4), containing 0.05% Tween 20, 0.1% BSA and 0.05% NaN3.

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact custom@neb.com for further information.

 

This product is related to the following categories:

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Storage Buffer

0.05% Tween® 20
0.02% NaN3
1 ml suspension in PBS Buffer
pH 7.4 @ 25°C

Support Matrix

1 µm nonporous superparamagnetic microparticle

Binding Capacity

5 μg Rabbit lgG/mg Goat Anti-Rabbit IgG Magnetic Beads

Can be Regenerated

Yes

Product Notes

  1. The amount of antibody required for optimal coating of the beads will vary with the PAb affinity and the antigen density on the cell surface. Optimal experimental conditions should be determined by titration on an individual basis.

Protocols, Manuals & Usage

Protocols

  1. Goat Anti-Rabbit IgG Magnetic Beads Protocol

FAQs & Troubleshooting

FAQs

  1. Do I need to block the beads or are they pre-blocked?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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