Product Class: Other

Chitin Magnetic Beads

Product Introduction

An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD).

  • Chitin beads have been prepared having a magnetite core, thereby permitting the magnetic isolation of CBD-fusion proteins from cell culture supernatants
  • The matrix can be regenerated without loss of binding capacity
  • Immobilized fusion proteins can be used in subsequent experiments to capture (pull down) target proteins from crude cell lysates that interact with the immobilized fusion protein
  • Binding capacity of 2 mg chitin binding domain protein/ml bed volume
  • Quick, small-scale purification of CBD-fusion proteins enables higher throughput and efficiency
  • Removal of CBD-tag during elution typically yields highly pure, native protein
Bioz Badge Exists : True
Catalog # Size Concentration
E8036S 5 ml
E8036L 25 ml

Product Information

Description

An affinity matrix for the small-scale isolation of target proteins fused to a chitin binding domain (CBD) (1). Chitin beads have been prepared having a magnetite core. This permits the magnetic isolation of CBD-fusion proteins from cell culture supernatants; after which, the matrix can be regenerated without loss of binding capacity. Immobilized fusion proteins can be used in subsequent experiments to capture target proteins from crude cell lysates that interact with the immobilized fusion protein.

IMPACT Citations

For larger volume requirements, customized and bulk packaging is available by purchasing through the OEM/Bulks department at NEB. Please contact the Customized Solutions team for further information.

This product is related to the following categories:
Chitin Purification (CBD-tag),
Affinity Purification,
Magnetic Beads and Racks,
Protein Purification,
This product can be used in the following applications:
Affinity Purification & Expression Tags,
Protein Purification,
High-throughput cloning and automation solutions,
IMPACT Affinity Tag

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Support Matrix

Approximately 50-70 μm paramagnetic microparticles

Binding Capacity

2 mg chitin binding domain protein / ml bed volume released

Can be Regenerated

Yes

Product Notes

  1. Do not freeze
  2. Efficiency of elution can be checked by eluting any protein that remains bound to the chitin magnetic beads with 50 µl of SDS-PAGE gel loading buffer and running 15 µl on a denaturing protein gel.

References

  1. Chong, S., Mersha, F. B., Comb, D. G., Scott, M. E., Landry, D., Vence, L. M., Perler, F. B., Benner, J., Kucera, R. B., Hirvonen, C. A., Pelletier, J. J., Paulus, H. and Xu, M.-Q. (1997). Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein-splicing element.. Gene. 192, 277-281.

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Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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