Product Introduction 3'-Desthiobiotin-GTP or 3'-Biotin GTP are guanosine triphosphate (GTP) analogs which are modified at their 3’ position with desthiobiotin or biotin, respectively. When used with the Vaccinia Capping System, (NEB #M2080) these reagents enable affinity tagging of RNA triphosphate ends. Tagged RNAs are enriched by binding to Hydrophilic Streptavidin Magnetic Beads (NEB #S1421). Desthiobiotin-tagged RNAs can be eluted with free biotin. This approach is used in Cappable-seq, a method developed at NEB for directly enriching the 5’-ends of primary transcripts (1). Bioz Badge Exists : True Catalog # Size Concentration N0761S 0.5 µmol 5 mM Featured Videos View Video Library Avoiding RNase Contamination