Product Class: Other

Quick-Load® Purple 50 bp DNA Ladder

Product Introduction

  • Convenient, ready-to-load format with NEB's proprietary purple loading dye
  • Size range: 50 bp to 1,350 bp
  • Sharp bands
  • No UV Shadow
  • Easy to identify reference bands
  • Supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
  • Suitable for 250 gel lanes

 

50 bp DNA Ladder
Bioz Badge Exists : True
Catalog # Size Concentration
N0556S 250 gel lanes (1.25 ml) 100 µg/ml

Product Information

Description

Quick-load Purple 50 bp DNA Ladder is a pre-mixed, ready-to-load molecular weight marker containing one tracking dye. Tracking dye does not leave a shadow under UV light.

The Quick-Load Purple 50 bp DNA Ladder consists of a proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for use as molecular weight standards for agarose gel electrophoresis. This digested DNA includes fragments ranging from 50 to 1,350 base pairs. The 200 and 500 bp bands have increased intensity to serve as reference points.

  • Recommended gel percentage range: 2-3.5%
  • Optimum separation on 3% 

 

Figure 1: Quick-Load Purple 50 bp DNA Ladder visualized

50 bp DNA Ladder

Quick-Load Purple 50 bp DNA Ladder visualized by ethidium bromide staining on a 3% TBE agarose gel.
Mass values are for 1 µg/lane.
Suggested Load: 5–10 µl/gel lane.
This product is related to the following categories:
Quick-Load Purple DNA Ladders,
DNA Markers & Ladders,
This product can be used in the following applications:
DNA Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Bases

Fragment Mass (ng) bp
1 103 1350
2 70 916
3 58 766
4 54 700
5 50 650
6 46 600
7 42 550
8 76 500
9 34 450
10 31 400
11 27 350
12 46 300
13 57 250
14 107 200
15 46 150
16 69 100
17 84 50

Effective Size Range

50bp to 1,350bp

Storage Buffer

2.5% Ficoll®-400
10 mM EDTA
3.3 mM Tris-HCl
0.001% Dye 2
0.02% Dye 1
pH 8 @ 25°C

Product Notes

  1. Preparation of DNA: The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.
  2. We recommend loading 5–10 μl (0.5–1 μg) of Quick-Load Purple 50 bp DNA Ladder per gel lane. In general, it is recommended to load 1 µl per mm of gel lane.
  3. The Quick-Load Purple 50 bp DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.
  4. Quick-Load Purple 50 bp DNA Ladder is stable for at least 6 months at 25°C. For long term storage, store at 4°C or -20°C. If stored at -20°C, mix well after thawing. If not well mixed, the load may float out of the wells.
  5. 1X Gel Loading Dye, Purple, no SDS: 
    2.5% Ficoll®-400
    10 mM EDTA
    3.3 mM Tris-HCl (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2

References

  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Molecular Cloning: A Laboratory Manual. (2nd ed.), pp.10.51-10.67, Cold Spring Harbor Laboratory Press.

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. Why are there extra bands visible on polyacrylamide gels?
  2. Can I use Midori Green with the DNA Ladders from NEB?
  3. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  4. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?
  5. Can I use GelRed® with the Low Molecular Weight and 50 bp DNA Ladders from NEB?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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