Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to incorporate 1 nmol ATP into an acid-insoluble material in 1 hour at 37°C.
Reaction Conditions
1X RNAPol Reaction Buffer
Supplement with 0.5 mM ATP, 0.5 mM GTP, 0.5 mM UTP and 0.5 mM CTP
Incubate at 37°C
1X RNAPol Reaction Buffer
40 mM Tris-HCl
6 mM MgCl2
1 mM DTT
2 mM spermidine
(pH 7.9 @ 25°C)
Storage Buffer
50 mM Tris-HCl
100 mM NaCl
20 mM β-ME
1 mM EDTA
50% Glycerol
0.1% (w/v) Triton® X-100
pH 7.9 @ 25°C
Unit Assay Conditions
1X RNAPol Reaction Buffer, supplemented with 0.5 mM each ATP, UTP, GTP, CTP, and 1 μg DNA containing the SP6 promoter in 50 μl.