Product Class: Other

NEBNext® Ultra™ End Repair/dA-Tailing Module

This product was discontinued on December 15, 2022. An alternative product is the NEBNext® Ultra™ II End Repair/dA-Tailing Module (NEB #E7546).

  • Catalog # E7442 was discontinued on December 15, 2022

Product Introduction

This module is part of the original Ultra™ workflow, and is optimized for use with the NEBNext® Ultra Ligation Module (NEB #E7445), for Illumina®-compatible library construction. The original Ultra workflow enables library preparation from 5 ng – 1 μg of input DNA in a streamlined protocol. 

However, we now recommend use of the more recent NEBNext Ultra II DNA workflow, which has been further optimized to provide increased yields of high quality libraries from a broader range of input amounts (500 pg – 1 μg), and a NEBNext Ultra II End Repair/dA-Tailing Module (NEB #E7546) is available.
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Catalog # Size Concentration

Product Information

Description






 

The NEBNext® Ultra™ End Repair/dA-Tailing Module is optimized to convert 5 ng–1 μg of fragmented DNA to repaired DNA having 5' phosphorylated, 3’ dA-tailed ends. The module is optimized for use with NEBNext Ultra Ligation Module (NEB #E7445), and is part of the original Ultra workflow, which enables library preparation from 5 ng – 1 μg of input DNA in a streamlined protocol. 

Lot Control

The lots provided are managed separately and qualified by additional functional validation. Individual reagents undergo standard enzyme activity and quality control assays, and also meet stringent criteria in the additional quality controls listed on each individual component page
This product is related to the following categories:
Next Generation Sequencing Library Preparation,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    E7442S -20
    Component Name Component # Stored at (°C) Amount Concentration
    NEBNext End Prep Enzyme Mix E7371AVIAL -20 1 x 0.072 ml Not Applicable
    NEBNext End Repair Reaction Buffer E7372AVIAL -20 1 x 0.156 ml 10 X

Properties & Usage

Materials Required but not Supplied

Magnetic rack or plate (e.g., NEBNext® Magnetic Separation Rack (NEB #S1515S), Alpaqua ® 96S Super Magnet Plate (#A001322), or equivalent)

FAQs & Troubleshooting

FAQs

  1. There are 2 NEBNext Ligation modules, the NEBNext Ultra Ligation Module (#E7445) and the NEBNext Quick Ligation Module(#E6056). Which module should be used in conjuction with the NEBNext Ultra End Repair/dA-Tailing Module?
  2. How do I clean up the reaction once complete?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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