Description
The Monarch Spin RNA Isolation Kit (Mini) is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from various biological samples, including cultured cells, mammalian tissues, microbes, plants, insects and blood. With just this single kit, users can purify up to 100 µg of high-quality total RNA from multiple sample types, from standard as well as tough-to-lyse samples, with included enzymes, reagents, and specialized gDNA removal columns. The kit uniquely enables binding capacities like RNA purification miniprep kits, combined with the low elution volumes of micro kits. Cleanup of enzymatic reactions and purification of RNA from TRIzol® -extracted samples is also possible using this kit. Purified RNA has metrics with A260/280 and A260/230 ratios typically > 2.0, high RNA integrity scores, and minimal residual gDNA. This kit captures RNA ranging in size from full-length rRNAs down to intact miRNAs. Purified RNA is suitable for downstream applications such as RT-qPCR, cDNA synthesis, RNA-seq, and RNA hybridization-based technologies.
Designed with sustainability in mind, Monarch kits use significantly less plastic than other kits on the market.
Properties
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Purification Format
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Spin column for nucleic acid purification
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Compatible Methods
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Centrifugation or vacuum manifold
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Intended Usage
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Total RNA isolation
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Compatible Sample Types
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Cells, microbes, plants, insects, mammalian tissues including soft, fibrous and lipid-rich, blood, plasma
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Yield
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Varies depending on sample type. Refer to Choosing Sample Input Types for RNA Purification and Average Yield Expected.
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Column Binding Capacity
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Up to 100 μg total RNA
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RNA Size
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≥ 20 nt, total RNA including small RNA, miRNA, mRNA, lncRNA, rRNA
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Elution Volume
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≥ 10 μl
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RNA Purity
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Typically A260/280 ≥ 2.0 and A260/230 ≥ 1.8
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Compatible
Downstream Applications
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cDNA synthesis, RT-qPCR, RNA-Seq, small RNA library prep, Northern blotting, hybridization-based workflows
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Figure 1: Overview of the Monarch Spin RNA Isolation Kit workflow
The Monarch Spin RNA Isolation Kit (Mini) enables total RNA purification from a wide range of sample types and elution in low volumes. The kit features unique spin columns designed with less plastic and includes reagents for optimal RNA extraction.
Figure 2: Monarch Spin RNA Isolation Kit (Mini) is a versatile solution for high-quality RNA extraction from a wide variety of sample types
RNA was extracted in duplicate using the Monarch Spin RNA Isolation Kit (Mini) from various samples representing a range of biological properties in the starting material, including fibrous, fatty and nuclease-rich mammalian tissues, tough-to-lyse samples such as Gram-positive bacteria, plants and insects, as well as RNA cleanup reactions. RNA quality was assessed using A260/A280 and A260/A230 ratios measured using a microvolume spectrophotometer (Lunatic®, Unchained Labs®). RNA integrity was measured using Agilent® automated electrophoresis systems according to sample types. For samples with known typical RNA profiles, Agilent RNA ScreenTape® was used on the TapeStation® 4200 (A). For samples with known atypical RNA profiles such as plant green tissue with plastidal content, and insects with ribosomal gene breaks, Agilent Bioanalyzer® 2100 used with NanoChip (B).
Figure 3: Monarch Spin RNA Isolation Kit (Mini) enables extraction of high-quality RNA even from low input samples
RNA was extracted using the Monarch Spin RNA Isolation Kit (Mini) from varying amounts of HEK293 cells over 5 orders of magnitude starting from 1 million cells to 100.
A. To assess RNA integrity, the extracted RNA was resolved using the Agilent® Pico Bioanalyzer chip on a Bioanalyzer 2100 with sample loading adjusted to the manufacturer's recommendation of assay input range.
B. To demonstrate successful downstream application, the RNA was subjected to RT-qPCR using Luna® Probe One-Step RT-qPCR 4X Mix with UDG (NEB #M3019) in a 20 μl reaction targeting GAPDH mRNA. Expected amplification curves were observed showing a quantitative trendcorresponding to the varying input amounts.
Figure 4: Monarch Spin RNA Isolation Kit (Mini) successfully purifies small RNAs below 200 nucleotides, enabling a more faithful representation of the total RNA pool compared to a leading supplier
RNA was extracted from 10 mg mouse brain tissues using Monarch Spin RNA Isolation Kit (Mini) and a comparable kit from Supplier Q. To visualize the isolation of small RNA species, the extracted RNA was resolved using the Agilent Small RNA chip on a Bioanalyzer 2100 according to manufacturer's instructions. Gel traces of RNA isolated from the two kits are shown (A), with representative electropherograms showing the small RNA size profiles (B).
Figure 5: Monarch Spin RNA Isolation Kit (Mini) offers greater flexibility in elution volumes with elution as low as 10 μl
RNA was extracted in replicate using the Monarch® Spin RNA Isolation Kit (Mini) with an input of 1 million HEK293 cells per extraction. To assess the recovery of RNA at varying elution volumes, RNA was eluted in 100, 50 30, 20 or 10 μl nuclease-free water. RNA concentration and subsequent yield were measured using a microvolume UV-Vis spectrophotometer (Lunatic®, Unchained Labs®). An elution volume of at least 20 μl is recommended to achieve maximal RNA recovery however, elution in as low as 10 μl can be applied depending on user's needs.
- This product is related to the following categories:
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Total RNA Extraction & Purification,
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RNA Extraction and Purification,
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Nucleic Acid Purification,