Description
The Monarch Spin Plasmid Miniprep Kit offers a unique method for plasmid preparation and purification, enabling extraction of highly concentrated plasmid DNA from various bacterial strains, with yields up to 20 μg of high-quality DNA. This new version features upgraded spin columns precision engineered to uniquely allow for low elution, in as little as 30 μl, for highly concentrated DNA in downstream application such as sequencing, restriction digestion, transformation, transfection, and more. A colored buffer system helps indicate completion of key lysis and neutralization steps for an easy to follow workflow and shorter, faster protocols allow for reduced hands-on time and less spin time. Compared to leading suppliers, these kits offer significantly less plastic through uniquely designed columns and bottles with plastic reduction while still maintaining structural integrity for centrifugation or vacuum manifolds and other steps. The kit features Monarch spin columns that are manufactured using state of the art automation machinery for high quality and consistency.
| Properties |
| Purification format |
Spin column for nucleic acid purification |
| Compatible methods |
Centrifugation or vacuum manifold |
| Intended Usage |
Plasmid extraction and preparation from various E.coli host strains and other microbial strains |
| Plasmid DNA Yield |
Up to 20 μg* |
| Culture volume |
1-5 ml, not to exceed 15 OD units |
| Column Binding Capacity |
Up to 20 μg |
| Plasmid Size |
Up to 25 kb |
| Elution Volume |
≥ 30 μl |
| DNA Purity |
A260/280 > 1.8 and A260/230 > 1.8 |
| Protocol Time |
9.5 - 12.5 minutes of spin and incubation time |
| Compatible Downstream Applications |
Restriction digestion and other enzymatic manipulations, transformation, transfection, DNA sequencing, PCR, labeling, cell-free protein synthesis, etc. |
* Yield depends on plasmid copy number, host strain, culture volume and growth conditions
Figure 1: Monarch Spin Plasmid Miniprep Kit consistently produces more concentrated plasmid DNA with equivalent or better yield and purity compared to the leading supplier
DNA concentration, yield and purity are higher with Monarch Spin Plasmid Miniprep Kit than leading supplier’s kit, across different plasmids. Preps were performed according to recommended protocols using ~1 ml (OD600 = 3) aliquot for pUC19 and pHT01 and ~2 ml (OD600 = 6) aliquot for Rluc and pXba of the same overnight cultures. Concentrations of plasmid were measured using a Trinean DropSense 16.
Figure 2: Plasmid DNA purified using Monarch Spin Plasmid Miniprep Kit are high-quality and suitable for downstream applications
Corresponding gel data shows purified plasmid DNA from Monarch Spin Plasmid Miniprep Kit works as well as leading supplier’s kit for downstream applications such as restriction digests. Gel data shows three replicates of plasmid purifications and subsequent restriction digests. Preps were performed according to recommended protocols using ~1 ml (OD600 = 3) aliquot for pUC19 and pHT01 and ~2 ml (OD600 = 6) aliquot for Rluc and pXba of the same overnight cultures. 1 μg of each prep was digested with XmnI (NEB #R0194) for pUC19, RLuc and pXba, and EcoRI (NEB #R0101) for pHT01 to linearize the plasmid and digests were resolved on 1.2% w/v TBE gel.
Figure 3: Monarch Spin Plasmid Miniprep Kit can elute in as low as 30 μl for high yield
Various elution volumes used in Monarch Spin Plasmid Miniprep Kit can generate high yield, highly concentrated DNA. 2 ml of NEB 10-beta Competent E. coli (NEB #C3019) transformed with pUC19 plasmid (NEB #N3041) was used. Monarch Buffer EY was used to elute the plasmid with the volumes indicated. Concentrations of plasmid were measured using a Trinean DropSense 16.
Figure 4: Features of the Monarch Spin Column S2D for Plasmid and Nucleic Acid Purification
NEB® Monarch’s unique column design and membrane assembly allows high-quality, highly-concentrated nucleic acid purification with low elution volume, for downstream applications. The column is designed and made with significantly less plastic for a reduced environmental impact.
Figure 5: Overview of the Monarch Spin Plasmid Miniprep Kit workflow
The Monarch Spin Plasmid Miniprep Kit features unique spin columns and optimized buffers based on alkaline lysis.
- This product is related to the following categories:
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Plasmid Purification,
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Nucleic Acid Purification,
- This product can be used in the following applications:
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Nucleic Acid Purification