Product Class: Other

3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog

Product Introduction

For most RNAs the cap structure increases stability, decreases susceptibility to exonuclease degradation, and promotes the formation of mRNA initiation complexes.

  • Also known as Anti-Reverse Cap Analog (ARCA)
  • Produces 100% translatable capped transcripts
  • Can be used for co-transcriptional capping with T7 (NEB #M0251), SP6 (NEB #M0207) and T3 RNA Polymerases
  • Can be used for synthesis of m7G capped RNA for in vitro splicing assays
  • Can be used for synthesis of m7G capped RNA for transfection or microinjection
  • Supplied as a dried sodium salt
Bioz Badge Exists : True
Catalog # Size Concentration
S1411S 1 µmol
S1411L 5 µmol

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Product Information

Description

Blocking of the 3´ -hydroxyl of m7G with 3´-0-Me assures that the capped transcripts are homogeneous. The 3´ - hydroxyl of the non-methylated G is the only 3´ - hydroxyl available for initiation (1).

The 5´terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (2,3,4). For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (5), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (5,6). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (6). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (7).

A method using E. coli RNA Polymerase primed with m7G(5´)ppp(5´)G or m7G(5´)ppp(5´)A for an efficient in vitro synthesis of capped RNAs has been developed by Contreas (8). Larger amounts of capped RNAs are produced by transcription systems using SP6 RNA Polymerase primed with m7G(5´)ppp(5´)G (7).

Figure 1: Structure of 3´-O-Me-m7G(5´)ppp(5´)G RNA Cap Structure Analog (NEB #S1411)

Structure of 3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog

Sequence

3'-O-Me-7mG(5')ppp(5')G
This product is related to the following categories:
RNA Capping,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    S1411S -20
    Component Name Component # Stored at (°C) Amount Concentration
    3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog S1411SVIAL -20 1 x 1 µmol Not Applicable
  • NEB # Stored at (°C)
    S1411L -20
    Component Name Component # Stored at (°C) Amount Concentration
    3´-O-Me-m7G(5')ppp(5')G RNA Cap Structure Analog S1411SVIAL -20 5 x 1 µmol Not Applicable

Properties & Usage

Supplied As

Dried Sodium Salt

Suggested Working Concentration

10mM

Recommended Resuspension Volume

100 μl

Product Notes

  1. Addition of 100 µl of water gives approximately a 10 mM solution.
  2. For cotranscriptional capping at a 4:1 ratio (mM) with GTP, resuspend in 25 ul of nuclease-free water for a 40 mM stock.

References

  1. Peng, Z.-H. et al. (2002). ORG.LETT.. 4(2), 161.
  2. Shatkin, A. J. (1978). Cell. 9, 645-653.
  3. Fillipowicz, W. (1978). FEBS Lett. 96, 1-11.
  4. Banerjee, A.K. (1980). Microbiol. Rev.. 44, 175-205.
  5. Miura, K. (1981). Adv. Biophys.. 14, 205-238.
  6. Shatkin, A. J. et al. (1977). Nucleic Acids. Res.. 4, 3065-3081.
  7. Konarska, M. M. et al. (1984). Cell. 38, 731-736.
  8. Contreas, R. et al. (1982). Nucleic Acids. Res.. 10, 6353-6363.
  9. Paterson, B. M. and Rosenberg, M. (1979). Nature. 279, 696-701.

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Specifications

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Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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