Product Class: Other

m7G(5')ppp(5')G RNA Cap Structure Analog

Product Introduction

 

  • The 5´terminal m7G cap present on most eukaryotic mRNAs promotes translation in vitro at the initiation level (1,2,3). 
  • A method for efficient in vitro synthesis of capped RNA using E.coli RNA polymerase primed with m7G(5´ )ppp(5´ )G (#S1404S) or m7G(5´ )ppp(5´ )A (#S1405S) has been developed by Contreas et al. (7).
Bioz Badge Exists : True
Catalog # Size Concentration
S1404S 1 µmol
S1404L 5 µmol

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Product Information

Description

For most RNAs, elimination of the cap structure causes a loss of stability, especially against exonuclease degradation (4), and a decrease in the formation of the initiation complex of mRNAs for protein synthesis (4,5). Certain prokaryotic mRNAs containing a 5´ terminal cap structure are translated as efficiently as or more efficiently than eukaryotic mRNAs in a eukaryotic cell-free protein synthesizing system (5). Also a cap requirement has been observed for splicing eukaryotic substrate RNAs (6).

 

Figure 1: Structure of m7G(5´)ppp(5´)G RNA Cap Structure Analog (NEB #S1404)

Structure of m7G(5')ppp(5')G RNA Cap Structure Analog

Sequence

7mG(5´)ppp(5´)G
This product is related to the following categories:
Nucleotide Solutions for RNA,
RNA Synthesis In vitro Transcription (IVT),

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    S1404S -20
    Component Name Component # Stored at (°C) Amount Concentration
    m7G(5')ppp(5')G RNA Cap Structure Analog S1404SVIAL -20 1 x 1 µmol Not Applicable
  • NEB # Stored at (°C)
    S1404L -20
    Component Name Component # Stored at (°C) Amount Concentration
    m7G(5')ppp(5')G RNA Cap Structure Analog S1404SVIAL -20 5 x 1 µmol Not Applicable

Properties & Usage

Supplied As

Dried Sodium Salt

Suggested Working Concentration

10mM

Recommended Resuspension Volume

100 μl

Product Notes

  1. Addition of 100 µl of water gives approximately a 10 mM solution.
  2. For cotranscriptional capping at a 4:1 ratio (mM) with GTP, resuspend in 25 ul of nuclease-free water for a 40 mM stock.

References

  1. Shatkin, A.J. (1978). Cell. 9, 645-653.
  2. Fillipowicz, W. (1978). FEBS Lett.. 96, 1-11.
  3. Banerjee, A.K. (1980). Microbiol. Rev.. 44, 175-205.
  4. Miura, K. (1981). Adv. Biophys.. 14, 205-238.
  5. Shatkin, A.J. et al. (1977). Nucl. Acids Res.. 4, 3065-3081.
  6. Konarska, M.M. et al. (1984). Cell. 38, 731-736.
  7. Contreas, R. et al. (1982). Nucl. Acids Res.. 10, 6353-2363.
  8. Paterson, B.M. and Rosenberg, M. (1979). Nature. 279, 696-701.

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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