Product Class: Restriction Endonuclease

BsmBI-v2
Gray property icon for NEB r3.1 Gray property icon for cloned at NEB recombinant engineered time saver Gray property icon for dilB 55 Gray property icon for heat inactivation 80° CpG

BsmBI-v2 has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10284618. Learn more.

This product replaces BsmBI (NEB #R0580).

Learn about Ligase Fidelity and Push the Limits of Golden Gate Assembly (50+ fragments).

NEB Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN and is optimized for Golden Gate Assembly
Isoschizomers | Single Letter Code

Product Introduction

 
Bioz Badge Exists : True
Catalog # Size Concentration
R0739S 200 units 10000 units/ml
R0739L 1000 units 10000 units/ml

Product Information

Description

 

Product Source

An E. coli strain that carries the modified cloned BsmBI gene from Bacillus stearothermophilus B61. BsmBI-v2 is an engineered and improved version of BsmBI. (NEB uses the designation "v2" or "HF" in the name to indicate engineered enzymes.)
This product is related to the following categories:
Restriction Endonucleases B,
Time-Saver Qualified Restriction Enzymes,
This product can be used in the following applications:
DNA Preparation,
DNA Analysis,
High-throughput cloning and automation solutions,
NEBridge® Golden Gate Assembly ,
Restriction Enzyme Digestion,
Restriction Enzyme Digestion

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 55°C in a total reaction volume of 50 µl.

Reaction Conditions

1X NEBuffer™ r3.1
Incubate at 55°C

1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Usage Concentration

1X

Activity in NEBuffers

NEBuffer™ r1.1: <10%
NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 25%

Diluent Compatibility

Storage Buffer

10 mM Tris-HCl
300 mM NaCl
1 mM DTT
0.1 mM EDTA
500 µg/ml Recombinant Albumin
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

80°C for 20 minutes

Methylation Sensitivity

dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked

Activity at Temperature

@37°C: 10%

Product Notes

  1. BsmBI-v2 is an isoschizomer of Esp3I

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Why is there a BsmBI site in LITMUS 38 but not LITMUS 39?
  2. Which restriction enzymes are used in Golden Gate Assembly?
  3. Which restriction enzymes are used in GoldenBraid Assembly?
  4. When is star activity a concern?
  5. How many nucleotides do I have to add adjacent to the RE recognition site in order to get efficient cutting?
  6. Which NEB restriction enzymes are supplied with Gel Loading Dye, Purple (6X)?
  7. Can you tell me more about the switch from BSA to Recombinant Albumin (rAlbumin) in NEBuffers?
  8. What are the differences between BsmBI-v2 and its isoschizomer, Esp3I?
  9. Is Gel Loading Dye, Purple (6X) or Gel Loading Dye, Purple (6X), no SDS compatible with other DNA binding dyes such as SYBR® and GelRed™ during gel electrophoresis?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

BsmBI-v2 has been reformulated with Recombinant Albumin (rAlbumin) beginning with Lot #10284618. For additional information, please visit at NEB Restriction Enzyme formulations with Recombinant Albumin (rAlbumin).

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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