Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to convert 1 µg of supercoiled plasmid pBR322 DNA to open circular form in 1 hour at 65°C in a total reaction volume of 50 µl. Reaction Conditions
1X NEBuffer™ r3.1
Incubate at 65°C
1X NEBuffer™ r3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: <10%
NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 100%
rCutSmart™ Buffer: 10%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
200 µg/ml BSA
pH 7.4 @ 25°C
Heat Inactivation
80°C for 20 minutes Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive