Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of pUC19 DNA in 1 hour at 25°C in a total reaction volume of 50 µl. Reaction Conditions
1X rCutSmart™ Buffer
Incubate at 25°C
1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 50%
NEBuffer™ r2.1: 50%
NEBuffer™ r3.1: 10%
rCutSmart™ Buffer: 100%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
250 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
0.15% Triton® X-100
10 mM TCEP
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutes Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive
Isoschizomers
FaeI
FatI
Hin1II
Hsp92II
NlaIII
Activity at Temperature
@37°C: 10%