Description
EagI has a High Fidelity version, EagI-HF® (NEB #R3505)
High Fidelity (HF) Restriction Enzymes have 100% activity in CutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 minutes with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design.
Product Source
An E. coli strain that carries the EagI gene from Enterobacter agglomerans (R. Morgan).
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Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of pXba DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Reaction Conditions
1X NEBuffer™ 3.1
Incubate at 37°C
1X NEBuffer™ 3.1
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
100 µg/ml BSA
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ 1.1: 10%
NEBuffer™ 2.1: 25%
NEBuffer™ 3.1: 100%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
500 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 8 @ 25°C
Heat Inactivation
65°C for 20 minutes Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked
Isoschizomers
XmaIII+