Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 50 µl. Reaction Conditions
1X NEBuffer™ EcoRI/SspI
Incubate at 37°C
1X NEBuffer™ EcoRI/SspI
100 mM Tris-HCl
50 mM NaCl
10 mM MgCl2
0.025% Triton® X-100
(pH 7.5 @ 25°C)
Activity in NEBuffers
NEBuffer™ r1.1: 50%
NEBuffer™ r2.1: 100%
NEBuffer™ r3.1: 50%
rCutSmart™ Buffer: 50%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
250 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
0.15% Triton® X-100
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutes Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Not Sensitive