Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to digest 1 µg of λ DNA (HindIII digest) in 1 hour at 37°C in a total reaction volume of 50 µl. Reaction Conditions
1X NEBuffer™ 2.1
Incubate at 37°C
1X NEBuffer™ 2.1
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl2
100 µg/ml BSA
(pH 7.9 @ 25°C)
Activity in NEBuffers
NEBuffer™ 1.1: 100%
NEBuffer™ 2.1: 100%
NEBuffer™ 3.1: 10%
Diluent Compatibility
Storage Buffer
10 mM Tris-HCl
250 mM NaCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
0.15% Triton® X-100
pH 7.4 @ 25°C
Heat Inactivation
65°C for 20 minutes Methylation Sensitivity
dam methylation: Not Sensitive
dcm methylation: Not Sensitive
CpG Methylation: Blocked by Some Combinations of Overlapping