Product Class: Other

Rapid PNGase F Antibody Standard

Product Introduction

Rapid PNGase F Antibody Standard is a murine anti-MBP monoclonal antibody, isotype IgG2a. This antibody standard can be used as a positive control for Rapid PNGase F.

  • Supplied glycerol-free for optimal use in downstream mass spectrometry workflows
  • Contains typical IgG glycosylation in a conserved Asn (#295) of the Fc region
  • Can also be used as a positive control for IdeZ Protease (IgG-specific) (NEB #P0770)
  • Optimal stability for up to 24 months
Bioz Badge Exists : True
Catalog # Size Concentration
P6043S 250 µg 5 mg/ml

Featured Videos

View Video Library
  • PNGase_OneStep_720

    One-step protocol for deglycosylation with Rapid PNGase F

  • PNGase_TwoStep_720

    Two-step protocol for deglycosylation with Rapid PNGase F

Product Information

Description




Supplied in
10 mM sodium phosphate (pH 7.0), 100 mM NaCl

Apparent Molecular Weight
IgG2a is comprised of two heavy chains which are each approximately 49 KDa, as well as two light chains which are each approximately 24.4 KDa.

This product is related to the following categories:
Glycoprotein Substrates
This product can be used in the following applications:
Glycan Sequencing,
Recombinant Glycoprotein Expression,
Glycoprotein Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Isotype

Mouse Isotype IgG2a

Source Species

murine

FAQs & Troubleshooting

FAQs

  1. Is the Rapid PNGase F Antibody Standard a monoclonal antibody? What cell type is used for production?
  2. What isotype of IgG is the Rapid PNGase F Antibody Standard?
  3. Is the Rapid PNGase F Antibody Standard glycosylated? If so, in what region?
  4. Is the Rapid PNGase F Antibody Standard tagged?
  5. What is the amino acid sequence of the Rapid PNGase F Antibody Standard? Where is the glycosylation site?
  6. What are the typical N-glycans found on the Rapid PNGase F Antibody Standard?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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