Product Class: Other

Fetuin

Product Introduction

Fetuin is a glycoprotein containing sialylated N-linked and O-linked glycans that can be used as a positive control for endoglycosidase enzymes that cleave both N-linked and O-linked carbohydrates.

  • Purified from fetal calf serum for simplified analysis 
  • Convenient downstream analysis by SDS-PAGE gel shift and/or mass spectrometry
  • Optimal stability for up to 24 months
Bioz Badge Exists : True
Catalog # Size Concentration
P6042S 500 µg 10 mg/ml

Product Information

Description


 
Fetuin is a glycoprotein containing sialylated N-linked and O-linked glycans that can be used as a positive control for endoglycosidase enzymes.

Product Source

Fetal Calf Serum
This product is related to the following categories:
Glycoprotein Substrates,
This product can be used in the following applications:
Expression Systems,
Glycan Sequencing,
Recombinant Glycoprotein Expression,
Glycoprotein Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Storage Buffer

1X Milli-Q water

Molecular Weight

Apparent: 48.4 kDa

Product Notes

  1. 500 μg is enough for approximately 20 reactions.
  2. Due to heterogeneous glycosylation; Fetuin runs as a doublet on an SDS-PAGE gel.

FAQs & Troubleshooting

FAQs

  1. What is a good endoglycosidase control substrate?
  2. Why is Fetuin a good control substrate for both O-Glycosidase, PNGase F and the Protein Deglycosylation Mix?
  3. What is a typical Fetuin deglycosylation reaction condition?
  4. Do detergents inhibit exoglycosidases/endoglycosidases?
  5. How much endoglycosidase should I use to deglycosylate Fetuin under native conditions?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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