Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to cleave > 95% of the carbohydrate from 10 µg Porcine Fibrinogen in 1 hour at 37°C in a total reaction volume of 10 µl. Reaction Conditions
1X GlycoBuffer 4
Incubate at 37°C
GlycoBuffer 4
50 mM sodium acetate
(pH 4.5 @ 25°C)
Storage Buffer
20 mM Tris-HCl
50 mM NaCl
1 mM EDTA
pH 7.5 @ 25°C
Heat Inactivation
65°C for 10 minutes Molecular Weight
Apparent: 38.8 kDa
Unit Assay Conditions
Two fold dilutions of Endo F3 are incubated with 10 µg Porcine Fibrinogen and 1X GlycoBuffer 4 in a 10 µl reaction. The reaction mix is incubated at 37°C for 1 hour. Separation of reaction products are visualized via SDS-PAGE.