Product Class: Other

α1-3,6 Galactosidase
Gray property icon for NEBU Gray property icon for cloned at NEB recombinant Gray property icon for incubation temperature 37° Gray property icon for heat inactivation 65° Gray property icon for rAlbumin

New Reaction Buffer

Product Introduction

α1-3,6 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of α1-3 and α1-6 linked galactose residues from oligosaccharides.

  • Recombinant enzyme with no detectable endoglycosidase or other exoglycosidase contaminating activities
  • Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
  • ≥95% purity, as determined by SDS-PAGE and intact ESI-MS
  • Optimal activity and stability for up to 24 months

 

Bioz Badge Exists : True
Catalog # Size Concentration
P0731S 100 units 4000 units/ml

Product Information

Description

 α1-3,6 Galactosidase is a highly specific exoglycosidase that catalyzes the hydrolysis of α1-3 and α1-6 linked D-galactopyranosyl residues from oligosaccharides.

Detailed Specificity: Specificity can vary depending on incubation time and branching structure.
Figure 1: Detailed specificity of α1-3,6 Galactosidase
Figure 1: Detailed specificity of α1-3, 6 Galactosidase
Reaction (B) contained 4 units of α1-3, 6 Galactosidase, 1X GlycoBuffer 1 and 1X BSA in a total reaction volume of 10 µl. Reaction (B) shows that branched fucose inhibits cleavage. Reaction (A) contained 24 units of α1-3, 6 Galactosidase and 100 units of Neuraminidase, followed by a heat kill at 65°C for 10 minutes and a 2 hour digestion with 16 units of β1-4 Galactosidase. The reaction in (B) contained 1X GlycoBuffer 1 and 1X BSA in a total reaction volume of 20 µl. The reactions were incubated at 37°C. Complete digestion of the α1-3, 6 Galactosidase was determined by an observation of complete transformation of the substrate in (A) to the non-reducing terminal N-acetylglucosamine tetra antennary oligosaccharide.

Product Source

Cloned from Xanthomonas manihotis and expressed in E. coli.
This product is related to the following categories:
Exoglycosidases
This product can be used in the following applications:
Glycan Sequencing,
Recombinant Glycoprotein Expression,
Glycoprotein Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    P0731S 4
    Component Name Component # Stored at (°C) Amount Concentration
    α1-3,6 Galactosidase P0731SVIAL 4 1 x 0.025 ml 4,000 units/ml
    GlycoBuffer 1 B1727SVIAL -20 1 x 1 ml 10 X
    Recombinant Albumin (Low-glycerol) B9203SVIAL -20 1 x 0.25 ml 10 mg/ml

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to cleave > 95% of the terminal, α-D-galactose from 1 nmol Galα1-3Galβ1-4Gal-7-amino-4-methyl-coumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 µl.

Reaction Conditions

1X GlycoBuffer 1
Supplement with 100 µg/ml Recombinant Albumin (Low-glycerol)
Incubate at 37°C

1X GlycoBuffer 1
5 mM CaCl2
50 mM sodium acetate
(pH 5.5 @ 25°C)

Storage Buffer

20 mM Tris-HCl
50 mM NaCl
1 mM EDTA
pH 7.5 @ 25°C

Heat Inactivation

65°C for 10 minutes

Molecular Weight

Apparent: 70 kDa

Unit Assay Conditions

Two fold serial dilutions of α1-3,6 Galactosidase are incubated with 1 nmol AMC-labeled substrate in 1X GlycoBuffer 1 and 1X rHSA in a 10 µl reaction. The reaction mix is incubated for 1 hour at 37°C. Separation of reaction products are visualized via thin layer chromatography (1).

Product Notes

  1. Avoid repeated freeze-thaw cycles.

References

  1. Wong-Madden, S.T. and Landry, D. (1995). Glycobiology. 5, 19-28.

FAQs & Troubleshooting

FAQs

  1. Can I use α1-3,6 Galactosidase in a double digest with other exoglycosidases and/or endoglycosidases?
  2. What is a good positive control for α1-3,6 Galactosidase?
  3. What are Glycosidases and their uses?
  4. How much exoglycosidase should be used?
  5. Why have the NEB Glycosidase enzymes changed reaction buffers? What are the new reaction buffers and can I still use an enzyme with its old buffer? Where can I find the composition of the old buffers?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

June 2, 2026, the shelf life has been extended from 1 year to 2 years at 4°C.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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