α1-6 Mannosidase

Product Introduction

α1-6 Mannosidase is a highly specific exoglycosidase that removes unbranched α1-6 linked mannose residues from oligosaccharides.

When used in conjunction with α1-2,3 Mannosidase, the α1-6 Mannosidase will cleave α1-6 mannose residues from branched carbohydrate substrates.
Recombinant enzyme with no detectable endoglycosidase or other exoglycosidase contaminating activities
Glycerol-free for optimal performance in HPLC and mass spectrometry analysis
≥95% purity, as determined by SDS-PAGE and intact ESI-MS
Optimal activity and stability for up to 24 months

Bioz Badge Exists : True

Catalog #	Size	Concentration
P0727S	800 units	40000 units/ml
P0727L	4000 units	40000 units/ml

Product Information

Description

α1-6 Mannosidase is a highly specific exoglycosidase that removes unbranched α1-6 linked D-mannopyranosyl residues from oligosaccharides (1,2). When used in conjunction with α1-2,3 Mannosidase, the α1-6 Mannosidase will cleave α1-6 Mannose residues from branched carbohydrate substrates.

Figure 1: Detailed specificity of α1-6 Mannosidase — All reactions contained 32 units of α1-2,3 Mannosidase (NEB #P0729 ), 40 units of α1-6 Mannosidase, 1X GlycoBuffer 1 and 1X rHSA In a total reaction volume of 10 µl. Reactions were incubated at 37°C. The substrate depicted in (E) will not cut to completion. If this structure exists in any substrate it will be impervious to cleavage by α1-6 Mannosidase. Note: When used alone, α1-6 Mannosidase will still act only on linear substrates. When used in conjunction with α1-2,3 Mannosidase, the α1-6 Mannosidase will cleave α1-6 Mannose residues from branched carbohydrate substrates.

All reactions contained 32 units of α1-2,3 Mannosidase (NEB #P0729 ), 40 units of α1-6 Mannosidase, 1X GlycoBuffer 1 and 1X rHSA In a total reaction volume of 10 µl. Reactions were incubated at 37°C. The substrate depicted in (E) will not cut to completion. If this structure exists in any substrate it will be impervious to cleavage by α1-6 Mannosidase. Note: When used alone, α1-6 Mannosidase will still act only on linear substrates. When used in conjunction with α1-2,3 Mannosidase, the α1-6 Mannosidase will cleave α1-6 Mannose residues from branched carbohydrate substrates.

Product Source

Cloned from Xanthomonas manihotis and expressed in E. coli.

This product is related to the following categories:: Exoglycosidases

This product can be used in the following applications:: Recombinant Glycoprotein Expression

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

NEB #			Stored at (°C)
P0727S			4

Component Name	Component #	Stored at (°C)	Amount	Concentration
α1-6 Mannosidase	P0727SVIAL	4	1 x 0.02 ml	40,000 units/ml
GlycoBuffer 1	B1727SVIAL	-20	1 x 1 ml	10 X
Recombinant Albumin (Low-glycerol)	B9203SVIAL	-20	1 x 0.25 ml	10 mg/ml

NEB #			Stored at (°C)
P0727L			4

Component Name	Component #	Stored at (°C)	Amount	Concentration
α1-6 Mannosidase	P0727LVIAL	4	1 x 0.1 ml	40,000 units/ml
GlycoBuffer 1	B1727SVIAL	-20	1 x 1 ml	10 X
Purified BSA	B9001SVIAL	-20	1 x 0.25 ml	10 mg/ml

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to cleave > 95% of the terminal α-D-mannose from 1 nmol of Manα1-6Manα1-6Man-7-amino-4-methyl-coumarin (AMC), in 1 hour at 37°C in a total reaction volume of 10 µl.

Reaction Conditions

1X GlycoBuffer 1
Supplement with 100 µg/ml Recombinant Albumin (Low-glycerol)
Incubate at 37°C

1X GlycoBuffer 1
5 mM CaCl₂
50 mM sodium acetate
(pH 5.5 @ 25°C)

Storage Buffer

50 mM NaCl
20 mM Tris-HCl
1 mM EDTA
pH 7.5 @ 25°C

Heat Inactivation

65°C for 10 minutes

Molecular Weight

Apparent: 51 kDa

Unit Assay Conditions

Two fold dilutions of α1‑6 Mannosidase are incubated with 1 nmol AMC-labeled substrate in 1X GlycoBuffer 1, supplemented with 100 µg/ml rHSA, in a 10 µl reaction. The reaction mix is incubated for 1 hour at 37°C. Separation of reaction products are visualized via thin layer chromatography (1).

Product Notes

p-nitrophenyl-α-D-mannopyranoside is NOT a substrate for this enzyme.
Avoid repeated freeze-thaw cycles.

References

Wong-Madden, S.T. and Landry, D. (1995). Glycobiology. 5, 19-28.

FAQs & Troubleshooting

FAQs

Tech Tips

Using 1-2 µl is a good starting point for a 1 hr incubation of 1µg of glycoprotein or 100 nM of oligosaccharide.

When used alone α1-6Man removes only unbranched α1-6 linked mannose residues from oligosaccharides.

p-nitrophenyl-a-D-Mannopyranoside (pNP-Man) is NOT a substrate for this enzyme.

Repeated freeze/thaw cycles may reduce activity. Recommended storage temp 4°C.

Citations & Technical Literature

Brochures

Citations

Additional Citations

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective 07/22/2026, the shelf life has been extended from 1 year to 2 years at 4˚C.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

α1-6 Mannosidase

GlycoBuffer 1

Purified BSA

Legal and Disclaimers

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

α1-6 Mannosidase

Product Introduction