Product Class: Other

Quick-Load® 1 kb Extend DNA Ladder

Product Introduction

  • Size range: 0.5 kb to 48.5 kb
  • Convenient, ready-to-load format
  • Formulated with bromophenol blue dye
  • Easy to identify reference bands
  • Suitable for 125 gel lanes
Quick- Load 1 kb Extend DNA Ladder

 

Bioz Badge Exists : True
Catalog # Size Concentration
N3239S 125 gel lanes (1.25 ml) 50 µg/ml

Product Information

Description

The Quick-Load 1 kb Extend DNA ladder is a pre-mixed, ready-to-load molecular weight DNA marker containing bromophenol blue dye as a tracking dye.

The DNA Ladder consists of proprietary plasmids and DNA molecules which are digested to completion with appropriate restriction enzymes to yield 13 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The digested DNA includes fragments ranging from 0.5 to 48.5 kilobases. The 3 kb fragment has increased intensity to serve as reference band.

  • Recommended gel and migration conditions: 0.5% 1X TBE agarose, 5V/cm (distance between electrodes), for 90 to 105 minutes

 

Figure 1: Quick-Load 1 kb visualized

Quick-Load 1 kb Extend DNA Ladder

Quick-Load 1 kb Extend DNA Ladder visualized by ethidium bromide staining on a 0.5% TBE agarose gel. Mass values are for 0.5 μg/lane.
This product is related to the following categories:
DNA Markers & Ladders
This product can be used in the following applications:
DNA Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    N3239S 4
    Component Name Component # Stored at (°C) Amount Concentration
    Quick-Load® 1 kb Extend DNA Ladder N3239SVIAL 4 1 x 1.25 ml 50 µg/ml

Properties & Usage

Bases

Fragment Mass (ng) bp
1 30 48,502
3 45 15,000
4 33 10,002
5 33 8,001
6 40 6,001
7 33 5,001
8 26 4,001
9 100 3,001
10 37 2,000
11 27 1,500
12 33 1,000
2a 15 20,066
2b 15 19,871
13a 18 517
13b 17 500

Preparation

The double-stranded DNA is digested to completion with appropriate restriction enzymes, phenol extracted and equilibrated in storage buffer.

Effective Size Range

500bp to 48,502bp

Storage Buffer

2.5% Ficoll®-400
11 mM EDTA
3.3 mM Tris-HCl
0.017% SDS
0.015% bromophenol blue
pH 8 @ 25°C

Product Notes

  1. Quick-Load 1 kb Extend DNA Ladder is stable for at least 6 months at 25°C.

    Store at 4°C. Do not store at -20°C.
  2. Usage Recommendation: We recommend loading 10 μl (0.5 μg) of Quick-Load 1 kb Extend DNA Ladder per gel lane. For optimum separation of the highest molecular weight DNA fragments, run on a 0.6% (1X TAE or 1X TBE) agarose gel at 3.5 V/cm (distance between electrodes) for 3 hours.

    In general, it is recommended to load 1 ul per mm of gel lane.

    The Quick-Load 1 kb Extend DNA Ladder is not intended for precise quantification of DNA mass but can be used for approximating the mass of DNA in comparably intense samples of similar size.

References

  1. Sambrook, J., Fritsch, E. F. and Maniatis, T. (1989). Cold Spring Harbor: Cold Spring Harbor Laboratory Press.(Ed.), Molecular Cloning: A Laboratory Manual. pp. 10.51-10.67.

FAQs & Troubleshooting

FAQs

  1. Why are the DNA ladders showing up on my Southern blot? What is the sequence or composition of the ladder bands?
  2. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

QUICK-LOAD® is a registered trademark of New England Biolabs, Inc.
FICOLL® is a registered trademark of GE Healthcare Bio-Sciences AB.

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