Product Class: Other

pBR322 DNA-MspI Digest

The large size of this product was discontinued on 12/31/2020. The small size will continue to be available.

Product Introduction

  • Size range: 9 bp - 622 bp
  • Supplied with free vial of Gel Loading Dye, Purple (6X), no SDS (NEB #B7025)
  • Small size suitable for 50 gel lanes
pBR322 DNA-MspI Digest Marker

 

Bioz Badge Exists : True
Catalog # Size Concentration
N3032S 50 gel lanes (0.05 ml) 1000 µg/ml

Product Information

Description

The MspI digest of pBR322 DNA yields 26 fragments. Suitable for use as molecular weight standards for agarose gel electrophoresis (1,2).

Comes supplied with 1 vial of Gel Loading Dye, Purple (6X), no SDS.

  • Recommended gel percentage range: 2-3.5%
  • Optimum separation on 3% 

 

Figure 1: pBR322 DNA-MspI Digest visualized

 

pBR322 DNA-MspI Digest Marker
pBR322 DNA-MspI Digest visualized by ethidium bromide staining on a 3% agarose gel. Suggested Load: 1 µg/ gel lane.
This product is related to the following categories:
DNA Markers & Ladders
This product can be used in the following applications:
DNA Analysis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Bases

Fragment Mass (ng) bp
1 143 622
2 121 527
3 93 404
4 70 307
5 55 242
6 55 238
7 50 217
8 46 201
9 44 190
10 41 180
11 74 160/160
12 68 147/147
13 28 123
14 25 110
15 21 90
16 17 76
17 15 67
18 16 34/34
19 12 26/26
20 3 15
21 4 9/9

Effective Size Range

9bp to 622bp

Storage Buffer

10 mM Tris-HCl
1 mM EDTA
pH 8 @ 25°C

Product Notes

  1. Dilute in TE or other buffer of minimal ionic strength. DNA may denature if diluted in dH2O.

    1X Gel Loading Dye, Purple, no SDS: 
    2.5% Ficoll®-400
    10mM EDTA
    3.3mM Tris-HCl (pH 8.0@25°C)
    0.02% Dye 1
    0.001% Dye 2

References

  1. Sutcliffe, J.G. (1978). Cold Spring Harbor Symp. Quant. Bio.. 43, 77-90.
  2. Peden, K.W.C. (1983). Gene. 22, 277-280.

FAQs & Troubleshooting

FAQs

  1. Why is the separation of the lower bands incomplete?
  2. What are the overhangs on the DNA ladder fragments? Can I end-label them using the T4 polynucleotide kinase (PNK)? What about the Klenow fragment?
  3. How can I quantify the amount of DNA in each band of a marker?
  4. Can I use Midori Green with the DNA Ladders from NEB?
  5. Can I use SYBR® and/or GelRed® dyes with the DNA Ladders from NEB?
  6. Why is my DNA Ladder floating out the wells? Why are there no bands visible in my DNA ladder gel lane?

Tech Tips

To make it ready-to-load, dilute in TE buffer instead of water.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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