Product Class: Other

DNA Gyrase (E. coli)
Gray property icon for NEBU Gray property icon for cloned at NEB recombinant Gray property icon for incubation temperature 37° Gray property icon for heat inactivation 65°

Product Introduction

Mechanism of DNA Gyrase (E. coli)

  • Type II Topoisomerase
  • Negatively supercoils relaxed closed circular dsDNA

Bioz Badge Exists : True
Catalog # Size Concentration
M7636S 600 units 30000 units/ml

Product Information

Description

DNA Gyrase (E. coli) is a Type II topoisomerase that catalyzes the introduction of negative supercoils in DNA in the presence of ATP. The Gyrase holoenzyme is a heterotetramer made up of 2 GyrA (97 kDa) subunits and 2 GyrB (90 kDa) subunits.

Product Source

An E. coli strain containing the cloned GyrA and GyrB genes.
This product is related to the following categories:
Other,
DNA Modifying Enzymes & Cloning Technologies,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    M7636S -20
    Component Name Component # Stored at (°C) Amount Concentration
    DNA Gyrase (E. coli) M7636SVIAL -20 1 x 0.02 ml 30,000 units/ml
    Gyrase Reaction Buffer B7636SVIAL -20 1 x 1 ml 5 X

Properties & Usage

Materials Required but not Supplied

Unit Definition

One unit is defined as the amount of enzyme required to convert 0.5 µg relaxed pUC19 plasmid to the supercoiled form in 1X Gyrase Reaction Buffer incubated for 15 minutes at 37°C in a total reaction volume of 30 µl.

Reaction Conditions

1X Gyrase Reaction Buffer
Incubate at 37°C

Heat Inactivation

65°C for 20 minutes

Features

  • Enhanced reaction buffer for improved activity
  • Stable formulation for storage at -20°C

Product Notes

  1. The reaction requires ATP and Mg2+ and is further stimulated by spermidine. The supplied Gyrase Reaction Buffer incorporates these essential components within an optimized formulation, thereby facilitating enhanced gyrase activity.
  2. Proprietary reaction buffer enhances gyrase activity. 

References

  1. Gellert, M et al (1976). PNAS. 73, 3872-3876.
  2. Higgins, N.P. et al. (1978). PNAS. 75, 1773–1777.

FAQs & Troubleshooting

FAQs

  1. Can DNA Gyrase (E. coli) (NEB #M7636) and 9°N™ Reverse Gyrase (NEB #M0200) create supercoils in both closed and open circular DNA?
  2. Can DNA Gyrase (E. coli) (NEB #M7636) and 9°N™ Reverse Gyrase (NEB #M0200) act on linear DNA?
  3. Are DNA Gyrase (E. coli) (NEB #M7636) and 9°N™ Reverse Gyrase (NEB #M0200) compatible with transformation or transfection workflows?
  4. Are there contaminants that inhibit DNA Gyrase (E. coli) (NEB #M7636) and 9°N™ Reverse Gyrase (NEB #M0200)?
  5. How should DNA treated with DNA Gyrase (E. coli) (NEB #M7636), Topoisomerase I (NEB #M0301), or 9°N™ Reverse Gyrase (NEB #M0200) be run on a gel?
  6. Is the linking number (ΔLk) or the superhelical density (σ) that results from the DNA Gyrase (E.coli) (NEB #M7636) or 9°N™ Reverse Gyrase (NEB #M0200) reaction fixed or random?
  7. Will DNA Gyrase (E. coli) (NEB #M7636) add additional negative supercoils in DNA that is already partially supercoiled?
  8. Does E.coli DNA Gyrase work in other NEBuffers?
  9. What is the difference between DNA Gyrase (E. coli) (NEB #M7636), Topoisomerase I (E. coli) (NEB #M0301), and 9°N™ Reverse Gyrase (NEB #M0200)?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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