Product Class: Other

Histone H3.3 Human, Recombinant
Gray property icon for cloned at NEB recombinant

  • Catalog # M2507 was discontinued on December 18, 2023

Product Introduction

Histone H3.3, an H3 variant that is found in all eukaryotes from yeast to human, is replication and cell cycle phase-independent and is the most common H3 in non-dividing cells. It has been shown to be enriched in covalent modifications associated with gene activation (4,5).

  • Highly pure, recombinant protein
  • Identity confirmed using Edman Degradation to sequence intact protein; mass confirmed by ESI-TOF MS
  • Tested for exonuclease, endonuclease and protease contamination
Bioz Badge Exists : True
Catalog # Size Concentration

Product Information

Description

Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer (1,2). It is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation.

  

Protein Sequence: 
ARTKQ TARKS TGGKA PRKQL ATKAA RKSAP STGGV KKPHR YRPGT VALRE IRRYQ KSTEL LIRKL PFQRL VREIA QDFKT DLRFQ SAAIG ALQEA SEAYL VGLFE DTNLC AIHAK RVTIM PKDIQ LARRI RGERA (Genbank accession number: P84243)

ESI-TOF Mass Spectrometry Analysis of Histone H3.3 Human, Recombinant
ESI-TOF Analysis of Histone H3.3 Human, Recombinant


SDS-PAGE analysis of histone H3.3 Human, Recombinant
SDS-PAGE analysis of histone H3.3 Human, Recombinant
Lane 1 & 7: NEB Protein Ladder (NEB #P7703),
Lane 2 thru 6: 0.5, 1.0, 2.0, 5.0, 10.0 µg Histone H3.3 Human, Recombinant

Product Source

An E. coli strain that carries a plasmid encoding the cloned human histone H3.3 gene, H3F3A or H3F3B. (Genbank accession number: AK311905)
This product is related to the following categories:
Discontinued (<3 years),

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    M2507S -20
    Component Name Component # Stored at (°C) Amount Concentration
    Histone H3.3 Human, Recombinant M2507SVIAL -20 1 x 0.1 ml Not Applicable

Properties & Usage

Storage Buffer

300 mM NaCl
1 mM EDTA
1 mM DTT
20 mM Sodium Phosphate
pH 7 @ 25°C

Molecular Weight

Theoretical: 15197 daltons

Product Notes

  1. 1 mg/ml (66 μM) is calculated using the molar extinction coefficient for Histone H3.3 (3960) and its absorbance at 280  nm (6,7).  1.0 A280 units = 3.8 mg/ml.
  2. Synonym: Histone H3.3A, H3F3, H3.3B.

References

  1. Kornberg, R.D. (1989). Annu. Rev. Biochem.. 46, 931-954.
  2. van Holde, K.E. (1989). Chromatin. 1-497,
  3. Gabrielli et al. (1984). Mol. Cell. Biochem.. 65, 57-66.
  4. Henikoff, S. et al. (2004). PNAS. 101, 1525-1530.
  5. Hake, S.B. et al. (2006). J. Biol. Chem.. 281, 559-568.
  6. Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem. 182, 319-326.
  7. Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.

FAQs & Troubleshooting

FAQs

  1. Are the histones fusion proteins or tagged proteins?
  2. Do the histones need to be reconstituted?
  3. What are the recommended histone storage conditions?
  4. Can the histones be used as substrates for protein modification enzymes? Which ones?

Tech Tips

After thawing on ice, mix well by pipetting the solution up and down. Do not centrifuge.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.