Histone H3.2 Human, Recombinant

Catalog # M2506 was discontinued on December 18, 2023

Product Introduction

Histone H3.2 is an H3 variant that is found in all eukaryotes, except budding yeast. It is replication-dependent and is associated with gene silencing.

Highly pure, recombinant protein
Identity confirmed using Edman Degradation to sequence intact protein; mass confirmed by ESI-TOF MS
Tested for exonuclease, endonuclease and protease contamination

Bioz Badge Exists : True

Product Information

Description

Histone H3 combines with Histone H4 to form the H3/H4 tetramer. Two H2A/H2B heterodimers interact with an H3/H4 tetramer to form the histone octamer. It is also modified by various enzymes and can act as a substrate for them. These modifications have been shown to be important in gene regulation.

Protein Sequence: ARTKQ TARKS TGGKA PRKQL ATKAA RKSAP ATGGV KKPHR YRPGT VALRE IRRYQ KSTEL LIRKL PFQRL VREIA QDFKT DLRFQ SSAVM ALQEA SEAYL VGLFE DTNLC AIHAK RVTIM PKDIQ LARRI RGERA (Genbank accession number: Q71DI3)

H3.2 SDS-PAGE analysis of Histone H3.2 Human, Recombinant — Lane 1 & 7: NEB Protein Ladder (NEB #P7703).
Lane 2 through 6: 0.5, 1.0, 2.0, 5.0, 10.0 μg Histone H3.2 Human, Recombinant

Product Source

An E.coli strain that carries a plasmid encoding the cloned human histone H3.2 gene, HIST2H3A or HIST2H3C. (Genbank accession number: BC130637)

This product is related to the following categories:

Properties & Usage

Storage Buffer

20 mM Sodium Phosphate
300 mM NaCl
1 mM DTT
1 mM EDTA
pH 7 @ 25°C

Molecular Weight

Theoretical: 15257 daltons

Product Notes

1 mg/ml, 66 µM is calculated using the molar extinction coefficient for Histone H3.2 (3960) and its absorbance at 280 nm (4,5). 1.0 A280 units = 3.9 mg/ml
Synonym: Histone H3/m, H3/o
Gene Synonym: H3F2, H3FM

References

Kornberg, R.D. (1977). Annu. Rev. Biochem.. 46, 931-954.
van Holde, K.E. (1989). Chromatin. 1-497.
Hake, S.B. et al. (2006). J. Biol. Chem.. 281, 559-568.
Gill, S.C. and von Hippel, P.H. (1989). Anal. Biochem.. 182, 319-326.
Pace, C.N. et al. (1995). Protein Science. 4, 2411-2423.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

M2506S_v2

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Histone H3.2 Human, Recombinant

Legal and Disclaimers

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This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.