Product Class: Other

ET SSB
recombinant Gray property icon for heat inactivation no

Product Introduction

ET SSB can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing.

  • Improve the processivity of DNA polymerase
  • Stabilization and marking of ssDNA structure
  • Increase the yield and specificitiy of PCR
  • Increase the yield and processivity of RT during RT-PCR
  • Improve DNA sequencing through regions with strong secondary structure
Bioz Badge Exists : True
Catalog # Size Concentration
M2401S 50 µg 500 µg/ml

Product Information

Description

ET SSB (Extreme Thermostable Single-Stranded DNA Binding Protein) is a single-stranded DNA binding protein isolated from a hyperthermophilic microorganism, which remains fully active after incubation at 95°C for 60 minutes. Due to the extreme thermostability, ET SSB can be used in applications that require extremely high temperature conditions, such as nucleic acid amplification and sequencing.

Figure 1: Improved multiplex PCR by ET SSB

ET SSB gel for multiplex PCR 

By adding ET SSB, specific amplification was accomplished in PCR using one to five primer pairs in incurring order (lanes 1-5). Lane 5C shows a PCR reaction using 5 primer pairs, with no ET SSB included (control reaction). Lane M: 100 bp DNA Ladder.

Figure 2: Multiplex HDA was improved by ET SSB

ET SSB gel for multiplex HDA 

The HDA reactions were prepared with either single or two sets of primers to amplify the invE (A) and/or invA (B) from Salmonella typhimurium genomic DNA. Lane M: Low Molecular DNA Ladder; Lane A: invE gene; Lane B: invA gene; Lane AB: invE and invA genes with no ET SSB present; Lane AB**: invE and invA genes with ET SSB in the HDA reaction. ** the reaction contained ET SSB.

Product Source

An E. coli strain that carries the cloned ssb gene from a hyperthermophilic organism
This product is related to the following categories:
ssDNA Binding Proteins,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

Sold by mass of pure protein as determined by OD280.

Storage Buffer

10 mM Tris-HCl
100 mM KCl
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C

Heat Inactivation

No

Molecular Weight

Apparent: 16 kDa

Product Notes

  1. ET SSB is active in any polymerase buffer. Add 200 ng of ET SSB per 50 µl reaction.

References

  1. Myers, T.W. and Romano, L.J. (1988). J. Biol. Chem. 263, 17006-17015.
  2. Reddy, M.S. (2000). Biochemistry. 39, 14250-14262.
  3. West, S.C., Cassuto, E. and Howard-Flanders, P. (1982). Mol. Gen. Genet. 186, 333-338.
  4. Goldmeyer, J., Kong, H., and Tang, W. (2007). J. Mol. Diagnostics. 9, 639-644.
  5. Delius, H., Mantell, N.J. and Alberts, B. (1972). J. Mol. Biol. 67, 341-350.
  6. Schwarz, K., Hansen-Hagge, T. and Bartram, C. (1990). Nucl. Acids Res. 18, 1079.
  7. Chou, Q. (1992). Nucl. Acids Res. 20, 4371.
  8. Oshima, R.G. (1992). Biotechniques. 13, 188.
  9. Rapley, R. (1994). Mol. Biotechnol. 2, 295-298.
  10. Olszewski, M. et al. (2005). Mol. Cell Probes. 19, 203-205.
  11. Baugh, L.R. et al. (2001). Nucl. Acids Res. 29, e29.
  12. Villalva, C. et al. (2001). Biotechniques. 31, 81-83, 86.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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