Product Class: Other

EnClose® TelN Protelomerase
Gray property icon for NEBU Gray property icon for cloned at NEB recombinant 30 75 Heat

This product will replace TelN Protelomerase (NEB #M0651).

Product Introduction

Mechanism of EnClose TelN Protelomerase, which forms covalently closed ends

  • Generate stable, covalently closed-ended DNA in a single enzymatic step
  • Retained His-tag supports downstream purification and QC method development
  • Precise, site-specific cutting at a 56 bp TelN recognition sequence
  • Higher concentration format available upon request via Custom Solutions for dbDNA workflows

Bioz Badge Exists : True
Catalog # Size Concentration
M0768S 350 units 5000 units/ml

Product Information

Description

EnClose TelN Protelomerase, a telomere resolvase from phage N15, cuts dsDNA at a 56 bp recognition sequence and leaves covalently closed ends at the site of cleavage through hairpin formation.

Mechanism of EnClose TelN Protelomerase, which forms covalently closed ends


Product Source:
An E. coli strain that carries a plasmid with the TelN gene from phage N15 and retains an N-terminal 8x His-tag.

This product is related to the following categories:
Closed-ended DNA & Protelomerases,
Other,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    M0768S -20
    Component Name Component # Stored at (°C) Amount Concentration

    EnClose TelN Protelomerase

    		 M0768SVIAL -20 1 x 0.07 ml 5,000 units/ml
    Protelomerase Reaction Buffer B0621SVIAL -20 1 x 1 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to cleave 0.5 μg of pMiniT-TelN BsaI-linearized control plasmid (313 fmol of TelN recognition sites) in a total reaction volume of 50 μl in 30 minutes at 30°C in 1X Protelomerase Reaction Buffer.

Reaction Conditions

1X Protelomerase Reaction Buffer
Incubate at 30°C

Protelomerase Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.001% Tween® 20
(pH 8.8 @ 25°C)

Heat Inactivation

75°C for 5 minutes

Features

  • His-tagged
  • Buffer free of substances of very high concern (SVHCs) as defined by REACH (Registration, Evaluation, Authorization and Restriction of Chemicals)

FAQs & Troubleshooting

FAQs

  1. Do EnClose® TelN Protelomerase (NEB #M0768) and TelN Protelomerase (NEB #M0651) digest TelN recognition sequences in supercoiled dsDNA (e.g., plasmid) and/or linear dsDNA?
  2. What is the structure at the cleavage site of the dsDNA after TelN Protelomerase digestion?
  3. What is the activity of EnClose® TelN Protelomerase (NEB #M0768) in common buffers?
  4. Are the recognition sequences for EnClose® TelA Protelomerase (NEB #M0621) or EnClose TelN Protelomerase (NEB #M0768) available as text or FASTA files?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In