Product Class: Other

Nuclease P1
Gray property icon for NEBU Gray property icon for incubation temperature 37° 75 Heat

Product Introduction

Mechanism of Nuclease P1


  • Single-stranded (ssDNA or RNA) specific endonuclease
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Nuclease P1 is ideal for:

  • Conversion of single-stranded DNA or RNA to 5'-mononucleotides
  • Analysis of the base composition of nucleic acids
  • Studies of the potential damage and modification in DNA
Bioz Badge Exists : True
Catalog # Size Concentration
M0660S 10000 units 100000 units/ml

Product Information

Description

Nuclease P1 is a zinc-dependent single-strand specific nuclease which hydrolyzes phosphodiester bonds in RNA and ssDNA with no base specificity. Nuclease P1 also exhibits 3′-phosphomonoesterase activity (1). Although a single-strand specific nuclease (ssDNA and RNA-specific), Nuclease P1 does display some activity toward dsDNA in Nuclease P1 Reaction Buffer. If preferentially degrading single-stranded nucleic acids (ssDNA or RNA) in the presence of double stranded DNA (dsDNA), we recommend using Nuclease P1 in 1X NEBuffer r1.1 to limit activity on dsDNA while maintaining single-strand nuclease activity.

Product Source

Isolated from Penicillium citrinum.
This product is related to the following categories:
Exonucleases and Non-specific Endonucleases,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    M0660S -20
    Component Name Component # Stored at (°C) Amount Concentration
    Nuclease P1 M0660SVIAL -20 1 x 0.1 ml 100,000 units/ml
    Nuclease P1 Reaction Buffer B0660SVIAL -20 2 x 1 ml 10 X

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to liberate 1.0 µg of acid soluble nucleotides from Torula Yeast total RNA per min at 37°C in 1X Nuclease P1 Reaction Buffer.

Reaction Conditions

1X Nuclease P1 Reaction Buffer
Incubate at 37°C

1X Nuclease P1 Reaction Buffer
50 mM sodium acetate
(pH 5.5 @ 25°C)

Storage Buffer

25 mM Tris-HCl
50 mM NaCl
1 mM ZnCl2
50% Glycerol
pH 7.2 @ 25°C

Heat Inactivation

75°C for 10 minutes

Product Notes

  1. Substrate specificity for Nuclease P1 is as follows: 3´ AMP > RNA > ssDNA >> dsDNA.
  2. The rate of hydrolysis of 2´-AMP is 3,000-fold less than that of 3´-AMP.
  3. Although a single-strand specific nuclease (ssDNA and RNA-specific), Nuclease P1 does display some activity toward dsDNA in Nuclease P1 Reaction Buffer. If preferentially degrading single-stranded nucleic acids (ssDNA or RNA) in the presence of double stranded DNA (dsDNA), we recommend using Nuclease P1 in 1X NEBuffer r1.1 to limit activity on dsDNA while maintaining single-strand nuclease activity.

References

  1. Fujimoto, M., Kuninaka, A. and Yoshino, H. (1974). Agric. Biol. Chem. 38, 777-783.

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Is Nuclease P1 sensitive to salt?
  2. Can Nuclease P1 be inactivated?
  3. Does Nuclease P1 require ZnCl2?
  4. What applications are Nuclease P1 best suited for?
  5. What substrates does Nuclease P1 digest?
  6. How much ssDNA or RNA can Nuclease P1 digest?
  7. What is the activity of Nuclease P1 in other buffers?
  8. How does the new unit definition relate to the historical unit definition for Nuclease P1?
  9. Will Nuclease P1 degrade double stranded DNA (dsDNA)?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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