Product Class: Other

EnClose® TelA Protelomerase
Gray property icon for NEBU Gray property icon for cloned at NEB recombinant 42 Gray property icon for heat inactivation 80°

Product Introduction

Mechanism of EnCloseTelA Protelomerase
  • EnClose TelA Protelomerase is a telomere resolvase
  • Cuts dsDNA at a minimal (26 bp) or full (50 bp) TelA recognition sequence
  • Leaves covalently closed ends through hairpin formation via tyrosine-mediated cleavage
  • Retains full activity using a minimal 26 bp recognition site.
    Bioz Badge Exists : True
    Catalog # Size Concentration
    M0621S 350 units 5000 units/ml

    Product Information

    Description

    EnClose TelA Protelomerase is a telomere resolvase that cuts dsDNA at a 50 bp recognition sequence and leaves covalently closed ends at the site of cleavage through hairpin formation. A minimal 26 bp sequence can also be utilized by the enzyme while retaining full activity.

    Full recognition site (50 bp) and minimal recognition site (26 bp) of EnCloseTelA Protelomerase

     

    Product Source:

    An E. coli strain that carries a recombinant plasmid encoding an engineered TelA Protelomerase from the Gram-negative bacteria Agrobacterium tumefaciens and retains an N-terminal 8x His-tag.
    This product is related to the following categories:
    Closed-ended DNA & Protelomerases,

    Reagents Supplied

    Reagents Supplied

    The following reagents are supplied with this product:

    NEB # Component Name Component # Stored at (°C) Amount Concentration
    • NEB # Stored at (°C)
      M0621S -20
      Component Name Component # Stored at (°C) Amount Concentration
      EnClose® TelA Protelomerase M0621SVIAL -20 1 x 0.07 ml 5,000 units/ml
      Protelomerase Reaction Buffer B0621SVIAL -20 1 x 1 ml 10 X

    Properties & Usage

    Unit Definition

    One unit is defined as the amount of enzyme required to cleave 0.5 µg of pMiniT2.0-TelA AhdI-linearized control plasmid (313 fmol of TelA recognition sites) in a total reaction volume of 50 µl in 30 minutes at 42°C in 1X Protelomerase Reaction Buffer.

    Reaction Conditions

    1X Protelomerase Reaction Buffer
    Incubate at 42°C

    Protelomerase Reaction Buffer
    20 mM Tris-HCl
    10 mM (NH4)2SO4
    10 mM KCl
    2 mM MgSO4
    0.001% Tween® 20
    (pH 8.8 @ 25°C)

    Heat Inactivation

    80°C for 10 minutes

    Features

    • Full activity on a minimal recognition sequence (26 bp)
    • Specific activity is higher than TelN Protelomerase
    • Buffer free of substances of very high concern (SVHCs) as defined by REACH (Registration, Evaluation, Authorization and Restriction of Chemicals)
    • His-tagged

    FAQs & Troubleshooting

    FAQs

    1. What is the activity of EnClose® TelA Protelomerase (NEB #M0621) in other common buffers?
    2. Are the recognition sequences for EnClose® TelA Protelomerase (NEB #M0621) or EnClose TelN Protelomerase (NEB #M0768) available as text or FASTA files?

    Quality, Safety & Legal

    Quality Assurance Statement

    Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    Specifications

    The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    Certificate Of Analysis

    The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    Safety DataSheets

    The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    Legal and Disclaimers

    Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

    This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

    New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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