Product Class: Other

Msz Exonuclease I
Gray property icon for rCutSmart Gray property icon for cloned at NEB recombinant 55 Gray property icon for heat inactivation 80°

Product Introduction

Mechanism for Msz Exonuclease I

  • DNA-specific exonuclease
  • Can be heat inactivated at 80°C in 1 minute
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Msz Exonuclease I (MszExoI) is Ideal for: 

  • Removal of linear single-stranded DNA oligos in thermophilic workflow applications within 45°C and 60°C.

  • This is an Enzyme for Innovation (EFI). EFI is a project initiated by NEB to provide unique enzymes to the scientific community in the hopes of enabling the discovery of new and innovative applications. These enzymes have interesting properties and unique specificities.
 
Bioz Badge Exists : True
Catalog # Size Concentration
M0527S 1000 units 10000 units/ml

Product Information

Description

Msz Exonuclease I is isolated from a strain of E. coli, which carries the cloned pMC45-1 gene from Methylocaldum szegediense. Msz Exonuclease I is a DNA specific exonuclease that catalyzes the removal of nucleotides from linear single-stranded DNA in the 3′ to 5′ direction with optimal activity between 45°C and 60°C in rCutSmart™ Buffer. 
This product is related to the following categories:
Exonucleases and Non-specific Endonucleases,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit of Msz Exonuclease I is defined as the amount of enzyme that will catalyze the release of 5 nmol of acid-soluble nucleotide in a total reaction volume of 100 µl in 15 minutes at 55°C in 1X rCutSmart Buffer.

Reaction Conditions

1X rCutSmart™ Buffer
Incubate at 55°C

1X rCutSmart™ Buffer
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
100 µg/ml Recombinant Albumin
(pH 7.9 @ 25°C)

Heat Inactivation

80°C for 1 minute

References

  1. Fei L, Tian S, Moysey R, Misca M, Barker JJ, Smith MA, et al. (2015). Structural and Biochemical Studies of a Moderately Thermophilic Exonuclease I from Methylocaldum szegediense. PLoS ONE. 10 (2), e0117470.

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. What is the optimal reaction temperature range for Msz Exonuclease I activity?
  2. Can Msz Exonuclease I remove primers in nested PCR reactions?
  3. Can Msz Exonuclease I be used to blunt dsDNA?
  4. Can Msz Exonuclease I be heat inactivated?
  5. Can Msz Exonuclease I be used with a double-stranded exonuclease to clean up plasmid preparations?
  6. Will Msz Exonuclease I work in other buffers?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective 9-5-23, unit definition changed.
 

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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