Product Class: Other

Exonuclease I (E. coli)
Gray property icon for NEBU recombinant Gray property icon for incubation temperature 37° Gray property icon for heat inactivation 80°

A thermolabile version is also available, Thermolabile Exonuclease I (NEB #M0568)

Product Introduction

Exonuclease I (E.coli), Thermostable Exonuclease I, Exonuclease T mechanism


  • DNA specific exonuclease
  • Catalyzes the removal of nucleotides from linear single-stranded DNA in the 3' to 5' direction
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

Exonuclease I is ideal for:

  • Removal of single-stranded primers in PCR reactions prior to Sanger DNA sequencing or SNP analysis
  • Removal of single-stranded primers for nested PCR reactions
  • Removal of linear single-stranded DNA, leaving behind double-stranded DNA in the sample

Note: See also Exo-CIP™ Rapid PCR Cleanup Kit (NEB #E1050).

Bioz Badge Exists : True
Catalog # Size Concentration
M0293S 3000 units 20000 units/ml
M0293L 15000 units 20000 units/ml

Product Information

Description

Highlights

  • Isolated from a recombinant source
  • 3' → 5' single strand exonuclease
  • Supplied with 10X Reaction Buffer

Product Source

An E. coli strain that carries the cloned Exo I gene from E. coli NM554.
This product is related to the following categories:
Exonucleases and Non-specific Endonucleases,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will catalyze the release of 10 nmol of acid-soluble nucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X Exonuclease I Reaction Buffer with 0.17 mg/ml single-stranded [3H]-DNA.

Reaction Conditions

1X Exonuclease I Reaction Buffer
Incubate at 37°C

1X Exonuclease I Reaction Buffer
67 mM Glycine-KOH
6.7 mM MgCl2
10 mM β-ME
(pH 9.5 @ 25°C)

Usage Concentration

10,000 units/ml

Storage Buffer

10 mM Tris-HCl
100 mM NaCl
5 mM β-ME
0.5 mM EDTA
100 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

80°C for 20 minutes

Application Features

  • Exonuclease I degrades excess single-stranded primer oligonucleotide from a reaction mixture containing double-stranded extension products

Product Notes

  1. Phosphorothioate (PT) bond is resistant to Exonuclease I (E. coli) cleavage. However, only one of the two chiral forms of the PT bond is resistant. To achieve complete protection against degradation at all PT bond positions, it is recommended to include five consecutive PT bonds in chemically synthesized DNA. Learn more here.

References

  1. Lehman and Nussbaum (1964). J. Biol. Chem. 239,
  2. Kusher, et al. (1971). Proc. Natl. Acad. Sci. USA. 68,
  3. Kusher et al. (1972). Proc. Natl. Acad. Sci. USA. 69, 1366.
  4. Goldmark and Linn (1972). J. Biol. Chem. 247,
  5. Rosamond et al. (1979). J. Biol. Chem. 254,

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. Can Exonuclease I be used to remove primers from amplification reactions?
  2. Will Exonuclease I degrade RNA?
  3. Can Exonuclease I be used to blunt DNA?
  4. Can Exonuclease I be heat inactivated?
  5. Will Exonuclease I work in other buffers?
  6. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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