Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme required to produce 1 nmol of ribonucleotides from 40 pmol of a fluorescently labeled 25 base pair RNA:DNA hybrid in a total reaction volume of 50 µl in 20 minutes at 50°C. Reaction Conditions
1X RNase H Reaction Buffer
Incubate at ≥50°C
1X RNase H Reaction Buffer
50 mM Tris-HCl
75 mM KCl
3 mM MgCl2
10 mM DTT
(pH 8.3 @ 25°C)
Storage Buffer
50 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
0.1% Triton® X-100
50% Glycerol
pH 7.5 @ 25°C
Heat Inactivation
No