Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 10 minutes at 37°C using poly(rA)•oligo(dT)18 as template. Reaction Conditions
1X ProtoScript® II Reverse Transcriptase Reaction Buffer
Incubate at 42°C
1X ProtoScript® II Reverse Transcriptase Reaction Buffer
50 mM Tris-HCl
75 mM KCl
3 mM MgCl2
(pH 8.3 @ 25°C)
Storage Buffer
20 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.01% IGEPAL® CA-630
pH 7.5 @ 25°C
Heat Inactivation
65°C for 20 minutes Unit Assay Conditions
50 mM Tris-HCl (pH 8.3), 75 mM KCl, 6 mM MgCl2, 10 mM dithiothreitol, 0.01% IGEPAL CA-630, 0.5 mM dTTP, 0.4 mM poly(rA)•oligo(dT)18.