Properties & Usage
Materials Required but not Supplied
- Nuclease-free Water (NEB #B1500)
- ThermoPol® Reaction Buffer (NEB #B9004) (decapping protocol only)
- EDTA
Unit Definition
One unit is the amount of enzyme that converts 1 µg 300 mer RNA transcript into a XRN-1 digestible RNA in 30 minutes at 37°C. Reaction Conditions
1X NEBuffer™ 2
Incubate at 37°C
1X NEBuffer™ 2
50 mM NaCl
10 mM Tris-HCl
10 mM MgCl2
1 mM DTT
(pH 7.9 @ 25°C)
Storage Buffer
20 mM Tris-HCl
200 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.01% Triton® X-100
pH 7.5 @ 25°C