Product Class: Other

Bst DNA Polymerase, Full Length
Gray property icon for NEBU Gray property icon for cloned at NEB recombinant 65 Gray property icon for heat inactivation 80°

The L size of this product has been discontinued.  The S size will still be available.

Product Introduction

Optimal performance in a number of applications

  • Isothermal DNA amplification
  • Primer extension
  • Utilizes ThermoPol® Reaction Buffer for optimum reaction conditions

Bioz Badge Exists : True
Catalog # Size Concentration
M0328S 500 units 5000 units/ml

Product Information

Description

Bst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5´ → 3´ polymerase and double-strand specific 5´ → 3´ exonuclease activity, but lacks 3´ → 5´ exonuclease activity (1).

Product Source

An E. coli strain that contains the gene from Bacillus stearothermophilus. (H. Kong)
This product is related to the following categories:
Isothermal Amplification & Strand Displacement
This product can be used in the following applications:
Isothermal Amplification

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

Reaction Conditions

1X ThermoPol® Reaction Buffer
Incubate at 65°C

1X ThermoPol® Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)

Storage Buffer

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.1 @ 25°C

Heat Inactivation

80°C for 20 minutes

5' - 3' Exonuclease

Yes

3' - 5' Exonuclease

No

Strand Displacement

No

Unit Assay Conditions

50 mM KCl, 20 mM Tris-HCl (pH 8.8), 10 mM MgCl2, 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24-mer, 200 µM dATP, 200 µM dCTP, 200 µM dGTP, 100 µM dTTP including [3H]-dTTP and 100 µg/ml BSA.

Product Notes

  1. Bst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.
  2. Reaction temperatures above 70°C are not recommended.
  3. Cannot be used for thermal cycle sequencing or PCR.

References

  1. Aliotta, J.M. et al. (1996). Genetic Analysis. 12, 185-195.
  2. Mead, D.A. et al. (1991). BioTechniques. 11, 76-87.

Tools & Resources

Selection Charts

FAQs & Troubleshooting

FAQs

  1. What are Hot Start and WarmStart® polymerases and when would I use them?

Troubleshooting

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specification Change Notifications

Effective March 15, 2022, RNase method incubation time has changed to 4 hours.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Trademarks

NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
TRITON® is a registered trademark of Union Carbide Corporation.

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