Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 55°C. Reaction Conditions
1X ThermoPol® Reaction Buffer
Incubate at 55°C
1X ThermoPol® Reaction Buffer
20 mM Tris-HCl
10 mM (NH4)2SO4
10 mM KCl
2 mM MgSO4
0.1% Triton® X-100
(pH 8.8 @ 25°C)
Storage Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7.4 @ 25°C
Heat Inactivation
No Molecular Weight
Theoretical: 40000 daltons
5' - 3' Exonuclease
No 3' - 5' Exonuclease
No Strand Displacement
No Unit Assay Conditions
1X ThermoPol Reaction Buffer, 200 µM dNTPs including [3H]-dTTP and 15 nM primed M13mp18. Activity at Temperature
@30°C: 5%
@37°C: 20%
@45°C: 40%
@65°C: 90%
@72°C: 70%