Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that catalyzes the conversion of 0.5 µg of UV irradiated supercoiled pUC19 DNA to > 95% nicked plasmid in a total reaction volume of 20 µl in 30 minutes at 37°C. Nicking is assessed by agarose gel electrophoresis. Irradiated plasmid contains an average of 3-5 pyrimidine dimers. Reaction Conditions
1X T4 PDG Reaction Buffer
Supplement with 100 µg/ml Recombinant Albumin, Molecular Biology Grade
Incubate at 37°C
1X T4 PDG Reaction Buffer
100 mM NaCl
1 mM DTT
1 mM EDTA
25 mM Na2HPO4
(pH 7.2 @ 25°C)
Storage Buffer
10 mM Tris-HCl
250 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.15% Triton® X-100
pH 7.4 @ 25°C
Heat Inactivation
No Unit Assay Conditions
1X T4 PDG Reaction Buffer containing 0.5 µg of UV irradiated supercoiled pUC19 DNA, supplemented with 100 µg/ml Recombinant Albumin in a 20 µl reaction.