Product Class: Other

T4 Gene 32 Protein
NEB4 Gray property icon for cloned at NEB recombinant Gray property icon for incubation temperature 37° Gray property icon for heat inactivation 65°

Available in Glycerol-free format. Please inquire.

Product Introduction

T4 Gene 32 Protein is a single-stranded DNA binding protein required for bacteriophage T4 replication and repair.

  • Used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structure
  • Increase yield and processivity of reverse transcriptase during RT-PCR
  • Increase yield and specificity of PCR products from soil samples
  • Used in Recombinase Polymerase Amplification (RPA)
Bioz Badge Exists : True
Catalog # Size Concentration
M0300S 100 µg 10 mg/ml
M0300L 500 µg 10 mg/ml

Product Information

Description

T4 Gene 32 Protein is a single-stranded DNA (ssDNA) binding protein required for bacteriophage T4 replication and repair (1-2). It cooperatively binds to and stablizes transiently formed regions of ssDNA and plays an important structural role during T4 phage replication (3). It also has been used extensively to stabilize and mark regions of ssDNA for electron microscopic examination of intracellular DNA structures (4-5). Recently, it has been shown to improve restriction enzyme digestion (6), improve the yield and efficiency of reverse transcription (RT) reactions during RT-PCR (7-9), enhance T4 DNA polymerase activity (10-11), as well as increase the yield of PCR products (12).

Product Source

An E. coli strain carrying a plasmid that overexpresses the gene 32 protein of T4 phage
This product is related to the following categories:
ssDNA Binding Proteins
This product can be used in the following applications:
Recombinase Polymerase Amplification and SIBA,
RT-PCR & cDNA Synthesis

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Reaction Conditions

1X NEBuffer™ 4
Incubate at 37°C

1X NEBuffer™ 4
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
1 mM DTT
(pH 7.9 @ 25°C)

Storage Buffer

20 mM Tris-HCl
100 mM NaCl
0.5 mM DTT
1 mM EDTA
50% Glycerol
pH 8 @ 25°C

Heat Inactivation

65°C for 20 minutes

Molecular Weight

Theoretical: 33506 daltons

Application Features

  • Stabilization and marking of ssDNA structures (4-5)
  • Increase yield and processivity of RT during RT-PCR (7-9)
  • Increase yield and specificity of PCR products from soil samples (10-12)

References

  1. Alberts, B. and Frey, L. (1970). Nature. 227, 1313-1318.
  2. Huberman, J.A. and Kornberg, A. (1971). J. Mol. Biol. 62, 39-52.
  3. Topal, M.D. and Sinha, N.K. (1983). J. Biol. Chem. 258, 12274-12279.
  4. Schwartz, K. et al. (1990). Nucl. Acids Res. 18, 1079.
  5. Kornberg, A. and Baker, T. DNA Replication. (2nd ed.), 328-332.
  6. Bittner, M. et al. (1979). J. Biol. Chem. 254, 9565-9572.
  7. Delius, H. et al. (1972). J. Mol. Biol.. 67, 341-350.
  8. Wu, M. and Davidson, N. (1975). Proc. Natl. Acad. Sci. USA. 72, 4506.
  9. Dombroski, D.F. and Morgan, A.R. (1985). J. Biol. Chem. 260, 415-447.
  10. Villalva, C. et al. (2001). Biotechniques. 31, 81-86.
  11. Baugh, L.R. et al. (2001). Nucl. Acids Res. 29, e29.
  12. Jeffries, D. and Farquharson, C. (2002). Anal Biochem. 308, 192-194.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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