Product Class: Other

Thermostable Inorganic Pyrophosphatase
recombinant Gray property icon for heat inactivation no

Product Introduction

Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate and can be used for the enhancement of DNA replication. This enzyme retains 100% activity after incubation at 100°C for 4 hours.
Bioz Badge Exists : True
Catalog # Size Concentration
M0296S 250 units 2000 units/ml
M0296L 1250 units 2000 units/ml

Product Information

Description

Inorganic pyrophosphatase (PPase) catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate:
P2O7-4 + H2O -> 2HPO4-2.

Product Source

An E. coli strain carrying a plasmid encoding a pyrophosphatase from the extreme thermophile Thermococcus litoralis.
This product is related to the following categories:
Phosphatases,
RNA Modification,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration
  • NEB # Stored at (°C)
    M0296S -20
    Component Name Component # Stored at (°C) Amount Concentration
    Thermostable Inorganic Pyrophosphatase M0296SVIAL -20 1 x 0.125 ml 2,000 units/ml
  • NEB # Stored at (°C)
    M0296L -20
    Component Name Component # Stored at (°C) Amount Concentration
    Thermostable Inorganic Pyrophosphatase M0296LVIAL -20 1 x 0.625 ml 2,000 units/ml

Properties & Usage

Unit Definition

One unit is the amount of enzyme that will generate 1 μmol of phosphate per minute from inorganic pyrophosphate under standard reaction conditions (a 10 minute reaction at 75°C in 50 mM Tricine [pH 8.5] , 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).

Storage Buffer

20 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8 @ 25°C

Heat Inactivation

No

Product Notes

  1. While a specific NEBuffer is not supplied, Thermostable Inorganic Pyrophosphatase works well in any buffer containing Mg++ (1-10 mM). This enzyme is typically used in PCR reactions, where the Mg++ concentration falls within this range.

References

  1. Heinonen, J.K. and Lahti, R.J. (1981). Analytical Biochemistry. 113, 313-317.

FAQs & Troubleshooting

FAQs

  1. What is the activity of TIPP at 37°C?

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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