Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate10 nmoles of dNTP into acid insoluble material in 30 minutes at 37°C. Reaction Conditions
1X T7 DNA Polymerase Reaction Buffer
Supplement with Recombinant Albumin, Molecular Biology Grade
Incubate at 37°C
1X T7 DNA Polymerase Reaction Buffer
20 mM Tris-HCl
10 mM MgCl2
1 mM DTT
(pH 7.5 @ 25°C)
Storage Buffer
50 mM KPO4
1 mM DTT
0.1 mM EDTA
50% Glycerol
pH 7 @ 25°C
Heat Inactivation
75°C for 20 minutes 5' - 3' Exonuclease
No 3' - 5' Exonuclease
Yes Strand Displacement
No Unit Assay Conditions
100 mM KCl, 20 mM Tris-HCl (pH 7.6), 6 mM MgCl2 , 0.5 mM DTT, 0.1 mM EDTA, 50 ug/ml Recombinant Albumin, 150 μM dNTPs including [3H]-dTTP and 70 µg/ml denatured herring sperm DNA. Error Rate
~ 15x10-6bases