Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 0.5 pmol of dNTP into acid insoluble material in 10 minutes at 30°C. Reaction Conditions
1X phi29 DNA Polymerase Reaction Buffer
Supplement with Recombinant Albumin, Molecular Biology Grade
Incubate at 30°C
1X phi29 DNA Polymerase Reaction Buffer
50 mM Tris-HCl
10 mM MgCl2
10 mM (NH4)2SO4
4 mM DTT
(pH 7.5 @ 25°C)
Storage Buffer
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.5% Tween® 20
0.5% IGEPAL® CA-630
pH 7.4 @ 25°C
Heat Inactivation
65°C for 10 minutes Molecular Weight
Theoretical: 67000 daltons
5' - 3' Exonuclease
No 3' - 5' Exonuclease
Yes Strand Displacement
++++ Unit Assay Conditions
1X phi29 DNA Polymerase Reaction Buffer, 0.1 mg/ml Recombinant Albumin, 0.01 mg/ml HindIII-digested λ DNA, 0.2 µM dTTP including [3H]-dTTP, 0.2 mM dGTP, 0.2 mM dATP and 0.2 mM dCTP.