Product Class: Other

T7 Exonuclease
NEB4 Gray property icon for cloned at NEB recombinant Gray property icon for incubation temperature 25° Gray property icon for heat inactivation no

Product Introduction

Diagram of mechanism


  • Double-stranded DNA specific exonuclease
  • Initiates at the 5' termini of linear or nicked double-stranded DNA
  • Catalyzes the removal of nucleotides from linear or nicked double-stranded DNA in the 5' to 3' direction
  • Need help finding the right exonuclease for your experiments? Try Exo Selector 

T7 Exonuclease is ideal for:

  • Site-directed mutagenesis
  • Nick-site extension 
Bioz Badge Exists : True
Catalog # Size Concentration
M0263S 1000 units 10000 units/ml
M0263L 5000 units 10000 units/ml

Product Information

Description

Highlights

  • Isolated from a recombinant source
  • 5' -> 3' exonuclease
  • Supplied with 10X Reaction Buffer

Product Source

Purified from an E. coli strain containing a TYB12 intein fusion.
This product is related to the following categories:
Exonucleases and Non-specific Endonucleases,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to produce 1 nmol of acid-soluble deoxyribonucleotide in a total reaction volume of 50 μl in 30 minutes at 37°C in 1X NEBuffer 4 with 0.15 mM sonicated duplex [3H]-DNA.

Reaction Conditions

1X NEBuffer™ 4
Incubate at 25°C

1X NEBuffer™ 4
50 mM Potassium Acetate
20 mM Tris-acetate
10 mM Magnesium Acetate
1 mM DTT
(pH 7.9 @ 25°C)

Activity in NEBuffers

NEBuffer™ 1: 30%
NEBuffer™ 2: 25%
NEBuffer™ 3: 1%
NEBuffer™ 4: 100%

Storage Buffer

10 mM Tris-HCl
5 mM DTT
0.1 mM EDTA
50% Glycerol
pH 8 @ 25°C

Heat Inactivation

No

Product Notes

  1. Phosphorothioate (PT) bond is resistant to T7 Exonuclease cleavage. However, only one of the two chiral forms of the PT bond is resistant. To achieve complete protection against degradation at all PT bond positions, it is recommended to include five consecutive PT bonds in chemically synthesized DNA. Learn more here.

References

  1. Kerr, C. and Sadowski, P.D. (1972). J. Biol. Chem. 247, 305-318.
  2. Shinozaki, K. and Okazaki, T. (1978). Nucl. Acids. Res. 5, 4245-4261.

Tools & Resources

Selection Charts

Web Tools

FAQs & Troubleshooting

FAQs

  1. How Does T7 Exonuclease differ from Lambda Exonuclease (NEB# M0262)?
  2. How Does T7 Exonuclease differ from Exonuclease III (NEB# M0206)?
  3. What is the activity of T7 Exonuclease in the NEBuffers?
  4. Can T7 Exonuclease be heat inactivated?
  5. What is the best way to generate partials using T7 Exonuclease?
  6. Can DNA be blunted using T7 Exonuclease?
  7. Will T7 Exonuclease work in rCutSmart buffer?
  8. Can Exonuclease I be used with a double stranded exonuclease to clean up plasmid preparations?

Tech Tips

Addition of 6 phosphorothioates to the 5’ end of DNA can block activity up to 90%.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

Other Products You May Be Interested In