Properties & Usage
Unit Definition
One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-insoluble material in a total reaction volume of 50 μl in 10 minutes at 37°C using poly(rA).oligo(dT) as template primer with 50 mM Tris-HCI (pH 8.3), 6 mM MgCl2, 10 mM dithiothreitol, 0.5 mM [3H]-dTTP and 0.4 mM poly(rA).oligo(dT)12-18. Reaction Conditions
1X M-MuLV Reverse Transcriptase Reaction Buffer
Incubate at 42°C
1X M-MuLV Reverse Transcriptase Reaction Buffer
50 mM Tris-HCl
75 mM KCl
3 mM MgCl2
10 mM DTT
(pH 8.3 @ 25°C)
Storage Buffer
50 mM Tris-HCl
150 mM NaCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% NP-40
pH 7.6 @ 25°C
Heat Inactivation
65°C for 20 minutes Molecular Weight
Theoretical: 78000 daltons