Product Class: Other

RNase If
NEB3 recombinant Gray property icon for incubation temperature 37° 70 Heat

Product Introduction

RNase If eliminates single-stranded RNA from DNA and protein preparations 

  • Isolated from a recombinant source
  • Supplied with 10X Reaction Buffer
  • Tested for the absence of DNA endonucleases and exonucleases 
  • Not sure which RNase to choose? Learn more about the different RNases offered by NEB.
Bioz Badge Exists : True
Catalog # Size Concentration
M0243S 5000 units 50000 units/ml
M0243L 25000 units 50000 units/ml

Product Information

Description

Ribonuclease If (RNase If) is an RNA endonuclease which will cleave at all RNA dinucleotide bonds leaving a 5´ hydroxyl
and 2´,3´ cyclic monophosphate (1). It has a preference for single-stranded RNA over double-stranded RNA in the absence of calcium (2). RNase If is a recombinant protein fusion of RNase I (from E. coli) and maltose-binding protein. It has identical activity to RNase I.

Product Source

An E. coli strain containing a genetic fusion of the E. coli RNase I gene (2nd) and the gene coding for maltose binding protein (MBP).
This product is related to the following categories:
RNases,
Epitranscriptome Analysis,

Reagents Supplied

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

Properties & Usage

Materials Required but not Supplied

  • Nuclease-free Water (NEB #B1500)
  • EDTA
  • Optional (for ethanol precipitation)
    • 3 M NaOAc (pH 5.5)
    • RNase-free Glycogen
    • Cold ≥ 95% Ethanol
    • 80% Ethanol

Unit Definition

One unit is defined as the amount of enzyme required to fully digest 1 picomole of synthetic ssRNA 33-mer in a total reaction volume of 10 µl in 15 minutes in 1X NEBuffer 3 as visualized on a 20% acrylamide gel.

Reaction Conditions

1X NEBuffer™ 3
Incubate at 37°C

1X NEBuffer™ 3
100 mM NaCl
50 mM Tris-HCl
10 mM MgCl2
1 mM DTT
(pH 7.9 @ 25°C)

Storage Buffer

10 mM Tris-HCl
100 mM NaCl
1 mM DTT
0.5 mM EDTA
50% Glycerol
pH 8 @ 25°C

Heat Inactivation

70°C for 20 minutes

Application Features

  • Degradation of single-stranded RNA to mono-, di- and trinucleotide (3)
  • Used in ribonuclease protection assays

Product Notes

  1. RNase If does not degrade DNA. It preferentially cleaves single-stranded RNA over double-stranded RNA. Digestion of dsRNA can be enhanced by the addition of Ca²⁺. RNase If can also degrade the RNA strand of RNA:DNA hybrid duplexes.

References

  1. Spahr, P.F. and Hollingworth, B.R. (1961). J. Biol. Chem.. 236, 823-831.
  2. Grünberg S, Coxam B, Chen TH, Dai N, Saleh L, Corrêa IR, Nichols NM, Yigit E. (2021). Nucleic Acids Res.. May 21;49(9), 5265-5277.
  3. Meador, J. III and Kennell, D. (1990). Gene. 95, 1-7.
  4. Meador, J. III, Cannon, B., Cannistraro, V.J. and Kennell, D. (1990). Eur. J. Biochem.. 187, 549-553.

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

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