AluI Methyltransferase

The L size of this product has been discontinued on 11/15/16. The S size will still be available.

Product Introduction

AluI Methyltransferase modifies the cytosine residue (C5) of the sequence AGCT.

Bioz Badge Exists : True

Catalog #	Size	Concentration
M0220S	100 units	5000 units/ml

Product Information

Description

Product Source

An E. coli strain that carries the cloned AluI modification gene from Arthrobacter luteus (ATCC 21606).

This product is related to the following categories:: Methyltransferases for Epigenetics,; Base Modifying Enzymes,

Reagents Supplied

The following reagents are supplied with this product:

NEB # Component Name Component # Stored at (°C) Amount Concentration

NEB #			Stored at (°C)
M0220S			-20

Component Name	Component #	Stored at (°C)	Amount	Concentration
AluI Methyltransferase	M0220SVIAL	-20	1 x 0.02 ml	5,000 units/ml
AluI Methyltransferase Reaction Buffer	B0220SVIAL	-20	1 x 1 ml	10 X
S-adenosylmethionine (SAM)	B9003SVIAL	-20	1 x 0.1 ml	32 mM

Properties & Usage

Unit Definition

One unit is defined as the amount of enzyme required to protect 1 µg of λ DNA in 1 hour at 37°C in a total reaction volume of 10 µl against cleavage by AluI restriction endonuclease.

Reaction Conditions

1X AluI Methyltransferase Reaction Buffer
Supplement with 640 µM S-adenosylmethionine (SAM)
Incubate at 37°C

Control Protection Assay

One unit of AluI Methyltransferase is incubated with 1 µg of λ DNA in 10 µl 1X AluI Methyltransferase Reaction Buffer, supplemented with 640 µM S-adenosylmethionine, for one hour at 37°C followed by 15 minutes inactivation at 65°C. The extent of protection by AluI Methyltransferase is determined by the addition of 10 units of AluI restriction endonuclease in 40 µl 1X NEBuffer 1 supplemented with 10 mM MgCl₂ followed by incubation at 37°C for 30 minutes. Results are analyzed on agarose gel.

Storage Buffer

10 mM Tris-HCl
50 mM KCl
1 mM DTT
0.1 mM EDTA
200 µg/ml BSA
50% Glycerol
pH 7.5 @ 25°C

Heat Inactivation

65°C for 15 minutes

References

Hoffman, J.L. (1986). Biochemistry. 25, 444-4449.

FAQs & Troubleshooting

FAQs

Tech Tips

Always add the SAM to the reaction buffer just before doing the methylation reaction, because the SAM is unstable in an aqueous solution.

Keep the SAM frozen at -20C for a longer shelf life, fresh SAM is important for optimal methylation reactions.

Citations & Technical Literature

Citations

Additional Citations

Quality, Safety & Legal

Quality Assurance Statement

Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

Specifications

The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

M0220S_v2

Certificate Of Analysis

The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

Safety DataSheets

The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

AluI Methyltransferase

AluI Methyltransferase Reaction Buffer

S-adenosylmethionine (SAM)

Legal and Disclaimers

Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

AluI Methyltransferase

Product Introduction